Synapsin II | HUABIO ET7109-88 product information

product summary

company name :

HUABIO

product type :

antibody

product name :

Synapsin II

catalog :

ET7109-88

quantity :

100μl

price :

385.00 USD

clonality :

monoclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

JE49-03

reactivity :

human, mouse, rat

application :

western blot, flow cytometry, immunohistochemistry - paraffin section

more info or order :

HUABIO product webpage

image

image 1 :

IF staining of Synapsin II in rat bone marrow (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.V

image 2 :

Immunofluorescence analysis of paraffin-embedded rat brain tissue labeling Synapsin II (ET7109-88) and GFAP (EM140707). The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS. And then probed with the primary antibodies Synapsin II (ET7109-88, red) at 1/200 dilution and GFAP (EM140707, green) at 1/400 dilution overnight at 4 ℃, washed with PBS. iFluor™ 594 conjugate-Goat anti-Rabbit IgG (HA1122) and iFluor™ 488 conjugate-Goat anti-Mouse IgG (HA1125) were used as the secondary antibodies at 1/1,000 dilution. DAPI was used as nuclear counterstain.

image 3 :

Immunofluorescence analysis of paraffin-embedded mouse brain tissue labeling Synapsin II (ET7109-88) and GFAP (EM140707). The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS. And then probed with the primary antibodies Synapsin II (ET7109-88, red) at 1/200 dilution and GFAP (EM140707, green) at 1/400 dilution overnight at 4 ℃, washed with PBS. iFluor™ 594 conjugate-Goat anti-Rabbit IgG (HA1122) and iFluor™ 488 conjugate-Goat anti-Mouse IgG (HA1125) were used as the secondary antibodies at 1/1,000 dilution. DAPI was used as nuclear counterstain.

product information

SKU :

ET7109-88

Target name :

Synapsin II

Species reactivity :

Mouse,Rat,Human

Applications :

WB,IF-Tissue,IHC-P,FC

Conjugate :

Non-conjugated

Immunogen :

Recombinant protein within Human Synapsin II aa 180-320 / 582.

Uniprot id :

Q92777>SwissProt: Q92777 Human;SwissProt: Q64332 Mouse;SwissProt: Q63537 Rat

Host :

Rabbit

Clone number :

JE49-03

Isotype :

IgG

Size :

100μl

List Price :

385.00 USD

Storage Buffer :

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Form :

Liquid

Storage Instruction :

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.

Purity :

Protein A affinity purified.

Product type :

Recombinant Rabbit monoclonal Antibody

Positive control :

Rat bone marrow tissue, rat brain tissue, mouse brain tissue, F9, rat cerebellum tissue.

Molecular wt :

Predicted band size: 63 kDa

Subcellular location :

Cell junction. Synapse.

Concentration :

1 mg/mL.

Recommended dilutions :

WB:1:500;IF-Tissue:1:50-1:200;IHC-P:1:50-1:200;FC:1:50-1:100

Pic img4 :

https://storage.huabio.cn/huabio/productImg/ET7109-88_4.jpg

Pic legend4 :

Immunohistochemical analysis of paraffin-embedded rat bone marrow tissue using anti-Synapsin II antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET7109-88, 1/200) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Pic img5 :

https://storage.huabio.cn/huabio/productImg/ET7109-88_5.jpg

Pic legend5 :

Immunohistochemical analysis of paraffin-embedded rat brain tissue using anti-Synapsin II antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET7109-88, 1/200) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Pic img6 :

https://storage.huabio.cn/huabio/productImg/ET7109-88_6.jpg

Pic legend6 :

Immunohistochemical analysis of paraffin-embedded mouse brain tissue using anti-Synapsin II antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET7109-88, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Pic img7 :

https://storage.huabio.cn/huabio/productImg/ET7109-88_7.jpg

Pic legend7 :

Flow cytometric analysis of Synapsin II was done on F9 cells. The cells were fixed, permeabilized and stained with Synapsin II antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). After incubation of the primary antibody on room temperature for an hour, the cells was stained with a Alexa Fluor™ 488-conjugated goat anti-rabbit IgG Secondary antibody at 1/500 dilution for 30 minutes.

Pic img8 :

https://storage.huabio.cn/huabio/productImg/ET7109-88_8.jpg

Pic legend8 :

Immunofluorescence analysis of paraffin-embedded rat cerebellum tissue labeling Synapsin II (ET7109-88) and GFAP (EM140707). The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS. And then probed with the primary antibodies Synapsin II (ET7109-88, red) at 1/200 dilution and GFAP (EM140707, green) at 1/400 dilution overnight at 4 ℃, washed with PBS. iFluor™ 594 conjugate-Goat anti-Rabbit IgG (HA1122) and iFluor™ 488 conjugate-Goat anti-Mouse IgG (HA1125) were used as the secondary antibodies at 1/1,000 dilution. DAPI was used as nuclear counterstain.

more info or order :

HUABIO product webpage