product summary
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company name :
HUABIO
product type :
antibody
product name :
Calpain small subunit 1
catalog :
ET7109-66
quantity :
100μl
price :
385.00 USD
clonality :
monoclonal
host :
domestic rabbit
conjugate :
nonconjugated
clone name :
JE47-72
reactivity :
human, mouse, rat
application :
western blot, flow cytometry, immunohistochemistry - paraffin section
more info or order :
image
image 1 :
Western blot analysis of Calpain small subunit 1 on different lysates with Rabbit anti-Calpain small subunit 1 antibody (ET7109-66) at 1/1,000 dilution.
Lane 1: 293T cell lysate (20 µg/Lane)
Lane 2: A549 cell lysate (20 µg/Lane)
Lane 3: Mouse lung tissue lysate (40 µg/Lane)
Lane 4: Rat lung tissue lysate (40 µg/Lane)
Predicted band size: 28 kDa
Observed band size: 28 kDa
Exposure time: 1 minute 40 seconds; ECL: K1802;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET7109-66) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
image 2 :
Immunohistochemical analysis of paraffin-embedded human liver cancer tissue using anti-Calpain small subunit 1 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET7109-66, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
image 3 :
Immunohistochemical analysis of paraffin-embedded human pancreas tissue using anti-Calpain small subunit 1 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET7109-66, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
product information
SKU :
ET7109-66
Target name :
Calpain small subunit 1
Species reactivity :
Human,Mouse,Rat
Applications :
WB,IHC-P,FC
Conjugate :
Non-conjugated
Immunogen :
Recombinant protein within Human Calpain small subunit 1 aa 1-140 / 268.
Uniprot id :
P04632>SwissProt: P04632 Human;SwissProt: O88456 Mouse;SwissProt: Q64537 Rat
Host :
Rabbit
Clone number :
JE47-72
Isotype :
IgG
Size :
100μl
List Price :
385.00 USD
Storage Buffer :
1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Form :
Liquid
Storage Instruction :
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.
Purity :
Immunogen affinity purified.
Product type :
Recombinant Rabbit monoclonal Antibody
Positive control :
293T cell lysate, A549 cell lysate, mouse lung tissue lysate, rat lung tissue lysate, human liver cancer tissue, human pancreas tissue, mouse liver tissue, mouse kidney tissue, rat uterus tissue, A549.
Molecular wt :
Predicted band size: 28 kDa
Subcellular location :
Cytoplasm. Membrane.
Concentration :
1 mg/mL.
Recommended dilutions :
WB: 1:500-1:2,000
;IHC-P: 1:50-1:200
;FC: 1:50-1:100
Pic img4 :
https://storage.huabio.cn/huabio/productImg/ET7109-66_4.jpg
Pic legend4 :
Immunohistochemical analysis of paraffin-embedded mouse liver tissue using anti-Calpain small subunit 1 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET7109-66, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Pic img5 :
https://storage.huabio.cn/huabio/productImg/ET7109-66_5.jpg
Pic legend5 :
Immunohistochemical analysis of paraffin-embedded mouse kidney tissue using anti-Calpain small subunit 1 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET7109-66, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Pic img6 :
https://storage.huabio.cn/huabio/productImg/ET7109-66_6.jpg
Pic legend6 :
Immunohistochemical analysis of paraffin-embedded rat uterus tissue using anti-Calpain small subunit 1 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET7109-66, 1/200) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Pic img7 :
https://storage.huabio.cn/huabio/productImg/ET7109-66_7.jpg
Pic legend7 :
Flow cytometric analysis of Calpain small subunit 1 was done on A549 cells. The cells were fixed, permeabilized and stained with the primary antibody (ET7109-66, 1/50) (red). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated Goat anti-Rabbit IgG Secondary antibody at 1/1000 dilution for 30 minutes.Unlabelled sample was used as a control (cells without incubation with primary antibody; black).
more info or order :
company information
HUABIO
Founded in 2007, HUABIO is dedicated to developing high-quality antibodies that advance innovation. We are passionate about the accuracy, efficiency, and consistency of our products. That is why we have invested in new production platforms, like recombinant rabbit monoclonals, alpaca nanobodies, and adopted aggressive QA standards to deliver cutting-edge antibodies with uncompromised quality.
We hope to see you at your next discovery!
We hope to see you at your next discovery!
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