BAG2 | HUABIO ET7109-62 product information

product summary

company name :

HUABIO

product type :

antibody

product name :

BAG2

catalog :

ET7109-62

quantity :

100μl

price :

385.00 USD

clonality :

monoclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

JE47-57

reactivity :

human

application :

western blot, flow cytometry

more info or order :

HUABIO product webpage

image

image 1 :

Western blot analysis of BAG2 on different lysates with Rabbit anti-BAG2 antibody (ET7109-62) at 1/500 dilution. Lane 1: HepG2 cell lysate Lane 2: Hela cell lysate Lane 3: Jurkat cell lysate Lane 4: A549 cell lysate Lane 5: K562 cell lysate Lysates/proteins at 10 µg/Lane. Predicted band size: 24 kDa Observed band size: 24 kDa Exposure time: 2 minutes; 12% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET7109-62) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:300,000 dilution was used for 1 hour at room temperature.

image 2 :

Flow cytometric analysis of BAG2 was done on Hela cells. The cells were fixed, permeabilized and stained with the primary antibody (ET7109-62, 1/100) (red). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated goat anti-rabbit IgG Secondary antibody at 1/500 dilution for 30 minutes.Unlabelled sample was used as a control (cells without incubation with primary antibody; black).

product information

SKU :

ET7109-62

Target name :

BAG2

Species reactivity :

Human

Applications :

WB,FC

Conjugate :

Non-conjugated

Immunogen :

Recombinant protein within Human BAG2 aa 1-118 / 211.

Uniprot id :

O95816>SwissProt: O95816 Human

Host :

Rabbit

Clone number :

JE47-57

Isotype :

IgG

Size :

100μl

List Price :

385.00 USD

Storage Buffer :

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Form :

Liquid

Storage Instruction :

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.

Purity :

Protein A affinity purified.

Product type :

Recombinant Rabbit monoclonal Antibody

Positive control :

HepG2 cell lysate, Hela cell lysate, Jurkat cell lysate, A549 cell lysate, K562 cell lysate, Hela.

Molecular wt :

Predicted band size: 24 kDa

Subcellular location :

Cytoskeleton, Cytosol.

Concentration :

1 mg/mL.

Recommended dilutions :

WB: 1:500-1:1,000 ;FC: 1:50-1:100

more info or order :

HUABIO product webpage