ATP2A1 / SERCA1 | HUABIO ET7109-43 product information

product summary

company name :

HUABIO

product type :

antibody

product name :

ATP2A1 / SERCA1

catalog :

ET7109-43

quantity :

100μl

price :

385.00 USD

clonality :

monoclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

JE45-75

reactivity :

human

application :

western blot, immunoprecipitation, immunohistochemistry - paraffin section

more info or order :

HUABIO product webpage

image

image 1 :

Western blot analysis of SERCA1 ATPase on human fetal skeletal muscle tissue lysate. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody was used at a 1:500 dilution in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.

image 2 :

Immunohistochemical analysis of paraffin-embedded human fetal skeletal muscle tissue using anti-SERCA1 ATPase antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the antibody (ET7109-43) at 1/200 dilution, for 30 minutes at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chrogen. Counter stained with hematoxylin and mounted with DPX.

image 3 :

Immunohistochemical analysis of paraffin-embedded human striated muscle tissue with Rabbit anti-ATP2A1 / SERCA1 antibody (ET7109-43) at 1/400 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET7109-43) at 1/400 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

product information

SKU :

ET7109-43

Target name :

ATP2A1 / SERCA1

Species reactivity :

Human

Applications :

WB,IP,IHC-P

Conjugate :

Non-conjugated

Immunogen :

Synthetic peptide within Human SERCA1 ATPase aa 1-50 / 1,001.

Uniprot id :

O14983>SwissProt: O14983 Human

Host :

Rabbit

Clone number :

JE45-75

Isotype :

IgG

Size :

100μl

List Price :

385.00 USD

Storage Buffer :

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Form :

Liquid

Storage Instruction :

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.

Purity :

Protein A affinity purified.

Product type :

Recombinant Rabbit monoclonal Antibody

Positive control :

Human fetal skeletal muscle tissue lysate, human fetal skeletal muscle tissue, human striated muscle tissue.

Molecular wt :

Predicted band size: 110 kDa

Subcellular location :

Endoplasmic reticulum membrane, Sarcoplasmic reticulum membrane.

Concentration :

1 mg/ml.

Recommended dilutions :

WB:1:500-1:1,000 ;IP: 1:10-1:50 ;IHC-P:1:50-1:400

more info or order :

HUABIO product webpage