Sarcomeric Alpha Actinin | HUABIO ET7109-42 product information

product summary

company name :

HUABIO

product type :

antibody

product name :

Sarcomeric Alpha Actinin

catalog :

ET7109-42

quantity :

100μl

price :

385.00 USD

clonality :

monoclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

JE45-22

reactivity :

human, mouse, rat

application :

western blot, immunoprecipitation, immunohistochemistry - paraffin section

more info or order :

HUABIO product webpage

image

image 1 :

Western blot analysis of Sarcomeric Alpha Actinin on different lysates with Rabbit anti-Sarcomeric Alpha Actinin antibody (ET7109-42) at 1/1,000 dilution. Lane 1: Mouse skeletal muscle tissue lysate Lane 2: Mouse heart tissue lysate Lane 3: Rat heart tissue lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 104 kDa Observed band size: 104 kDa Exposure time: 3 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET7109-42) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:50,000 dilution was used for 1 hour at room temperature.

image 2 :

Immunohistochemical analysis of paraffin-embedded rat heart tissue using anti-Sarcomeric Alpha Actinin antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the antibody (ET7109-42) at 1/200 dilution, for 30 minutes at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chrogen. Counter stained with hematoxylin and mounted with DPX.

image 3 :

Immunohistochemical analysis of paraffin-embedded human colon tissue using anti-Sarcomeric Alpha Actinin antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the antibody (ET7109-42) at 1/200 dilution, for 30 minutes at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chrogen. Counter stained with hematoxylin and mounted with DPX.

product information

SKU :

ET7109-42

Target name :

Sarcomeric Alpha Actinin

Species reactivity :

Human,Mouse,Rat

Applications :

WB,IP,IHC-P

Conjugate :

Non-conjugated

Immunogen :

Recombinant protein within human Sarcomeric Alpha Actinin aa 1-150.

Uniprot id :

P35609>SwissProt: P35609 Human;SwissProt: Q9JI91 Mouse;Entrez Gene:291245 Rat

Host :

Rabbit

Clone number :

JE45-22

Isotype :

IgG

Size :

100μl

List Price :

385.00 USD

Storage Buffer :

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Form :

Liquid

Storage Instruction :

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.

Purity :

Protein A affinity purified.

Product type :

Recombinant Rabbit monoclonal Antibody

Positive control :

Mouse skeletal muscle tissue lysate, mouse heart tissue lysate, rat heart tissue lysate, rat heart tissue, human colon tissue, human fetal skeletal muscle tissue, mouse heart tissue, mouse smooth muscle tissue.

Molecular wt :

Predicted band size: 104 kDa

Subcellular location :

Cytoplasm

Concentration :

1 mg/ml.

Recommended dilutions :

WB:1:500 ;IP:1:10-1:50 ;IHC-P:1:50-1:200

Pic img4 :

https://storage.huabio.cn/huabio/productImg/ET7109-42_4.jpg

Pic legend4 :

Immunohistochemical analysis of paraffin-embedded human fetal skeletal muscle tissue using anti-Sarcomeric Alpha Actinin antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the antibody (ET7109-42) at 1/200 dilution, for 30 minutes at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chrogen. Counter stained with hematoxylin and mounted with DPX.

Pic img5 :

https://storage.huabio.cn/huabio/productImg/ET7109-42_5.jpg

Pic legend5 :

Immunohistochemical analysis of paraffin-embedded mouse heart tissue using anti-Sarcomeric Alpha Actinin antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the antibody (ET7109-42) at 1/200 dilution, for 30 minutes at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chrogen. Counter stained with hematoxylin and mounted with DPX.

Pic img6 :

https://storage.huabio.cn/huabio/productImg/ET7109-42_6.jpg

Pic legend6 :

Immunohistochemical analysis of paraffin-embedded mouse smooth muscle tissue with Rabbit anti-Sarcomeric Alpha Actinin antibody (ET7109-42) at 1/100 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET7109-42) at 1/100 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

more info or order :

HUABIO product webpage