RPA32/RPA2 | HUABIO ET7109-41 product information

product summary

company name :

HUABIO

product type :

antibody

product name :

RPA32/RPA2

catalog :

ET7109-41

quantity :

100μl

price :

385.00 USD

clonality :

monoclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

JE45-59

reactivity :

human, mouse, rat

application :

western blot, immunoprecipitation, immunohistochemistry - paraffin section

more info or order :

HUABIO product webpage

image

image 1 :

Western blot analysis of RPA32/RPA2 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody was used at a 1:500 dilution in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: Mouse testis tissue lysate Lane 2: A431 cell lysate Lane 3: SH-SY-5Y cell lysate Lane 4: SiHa cell lysate Lane 5: 293 cell lysate Lane 6: Hela cell lysate Lane 7: HepG2 cell lysate

image 2 :

Immunohistochemical analysis of paraffin-embedded rat brain tissue using anti-RPA32/RPA2 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the antibody (ET7109-41) at 1/50 dilution, for 30 minutes at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chrogen. Counter stained with hematoxylin and mounted with DPX.

image 3 :

Immunohistochemical analysis of paraffin-embedded human colon tissue using anti-RPA32/RPA2 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the antibody (ET7109-41) at 1/200 dilution, for 30 minutes at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chrogen. Counter stained with hematoxylin and mounted with DPX.

product information

SKU :

ET7109-41

Target name :

RPA32/RPA2

Species reactivity :

Human,Mouse,Rat

Applications :

WB,IP,IHC-P,IF-Cell

Conjugate :

Non-conjugated

Immunogen :

Synthetic peptide within Human RPA32 / RPA2 aa 221-270 / 270.

Uniprot id :

P15927>SwissProt: P15927 Human;SwissProt: Q62193 Mouse;SwissProt: Q63528 Rat

Host :

Rabbit

Clone number :

JE45-59

Isotype :

IgG

Size :

100μl

List Price :

385.00 USD

Storage Buffer :

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Form :

Liquid

Storage Instruction :

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.

Purity :

Protein A affinity purified.

Product type :

Recombinant Rabbit monoclonal Antibody

Positive control :

Mouse testis tissue, A431, SH-SY-5Y, SiHa, 293, Hela, HepG2, rat brain tissue, human colon tissue, human breast cancer tissue, human kidney tissue.

Molecular wt :

Predicted band size: 29 kDa

Subcellular location :

Nucleus.

Concentration :

1 mg/ml.

Recommended dilutions :

WB: 1:500-1:2,000 ;IP: 1:10-1:50 ;IHC-P: 1:50-1:200 ;IF-Cell: 1:100

Advanced Validation :

Cell treatment (CT)

Pic img4 :

https://storage.huabio.cn/huabio/productImg/ET7109-41_4.jpg

Pic legend4 :

Immunohistochemical analysis of paraffin-embedded human breast cancer tissue using anti-RPA32/RPA2 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the antibody (ET7109-41) at 1/200 dilution, for 30 minutes at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chrogen. Counter stained with hematoxylin and mounted with DPX.

Pic img5 :

https://storage.huabio.cn/huabio/productImg/ET7109-41_5.jpg

Pic legend5 :

Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-RPA32/RPA2 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the antibody (ET7109-41) at 1/200 dilution, for 30 minutes at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chrogen. Counter stained with hematoxylin and mounted with DPX.

Pic img6 :

https://storage.huabio.cn/huabio/productImg/ET7109-41_6.jpg

Pic legend6 :

Immunohistochemical analysis of paraffin-embedded mouse testis tissue using anti-RPA32/RPA2 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the antibody (ET7109-41) at 1/200 dilution, for 30 minutes at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chrogen. Counter stained with hematoxylin and mounted with DPX.

Pic img7 :

https://storage.huabio.cn/huabio/productImg/ET7109-41_7.jpg

Pic legend7 :

Species: Human Cell sample: HeLa Antibody concentration: 1: 100 Date by conrtesy of: Mr. Haihua Xie School of Basic Medical Sicences, Zhejiang University

more info or order :

HUABIO product webpage