FYB | HUABIO ET7109-38 product information

product summary

company name :

HUABIO

product type :

antibody

product name :

FYB

catalog :

ET7109-38

quantity :

100μl

price :

385.00 USD

clonality :

monoclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

JE44-80

reactivity :

human, mouse, rat

application :

western blot, flow cytometry, immunohistochemistry - paraffin section

more info or order :

HUABIO product webpage

image

image 1 :

Western blot analysis of FYB on different lysates with Rabbit anti-FYB antibody (ET7109-38) at 1/5,000 dilution. Lane 1: U-937 cell lysate (15 µg/Lane) Lane 2: Jurkat cell lysate (15 µg/Lane) Lane 3: RAW264.7 cell lysate (15 µg/Lane) Lane 4: Mouse spleen tissue lysate (30 µg/Lane) Predicted band size: 85 kDa Observed band size: 110 kDa Exposure time: 2 minutes; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET7109-38) at 1/5,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

image 2 :

Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-FYB antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the antibody (ET7109-38) at 1/200 dilution, for 30 minutes at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chrogen. Counter stained with hematoxylin and mounted with DPX.

image 3 :

Immunohistochemical analysis of paraffin-embedded human appendix tissue using anti-FYB antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the antibody (ET7109-38) at 1/200 dilution, for 30 minutes at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chrogen. Counter stained with hematoxylin and mounted with DPX.

product information

SKU :

ET7109-38

Target name :

FYB

Species reactivity :

Human,Mouse,Rat

Applications :

WB,IHC-P,FC,IF-Cell

Conjugate :

Non-conjugated

Immunogen :

Synthetic peptide within Human FYB aa 701-750 / 783.

Uniprot id :

O15117>SwissProt: O15117 Human;SwissProt: O35601 Mouse;SwissProt: D3ZIE4 Rat

Host :

Rabbit

Clone number :

JE44-80

Isotype :

IgG

Size :

100μl

List Price :

385.00 USD

Storage Buffer :

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Form :

Liquid

Storage Instruction :

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.

Purity :

Protein A affinity purified.

Product type :

Recombinant Rabbit monoclonal Antibody

Positive control :

U-937 cell lysate, Jurkat cell lysate, RAW264.7 cell lysate, mouse spleen tissue lysate, human tonsil tissue, human appendix tissue, human spleen tissue, rat spleen tissue, HepG2.

Molecular wt :

Predicted band size: 85 kDa

Subcellular location :

Cell junction, Cytoplasm, Nucleus.

Concentration :

1 mg/mL.

Recommended dilutions :

WB: 1:5,000 ;IHC-P: 1:50-1:200 ;FC: 1:50-1:100 ;IF-Cell: 1:100

Pic img4 :

https://storage.huabio.cn/huabio/productImg/ET7109-38_4.jpg

Pic legend4 :

Immunohistochemical analysis of paraffin-embedded human spleen tissue using anti-FYB antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the antibody (ET7109-38) at 1/200 dilution, for 30 minutes at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chrogen. Counter stained with hematoxylin and mounted with DPX.

Pic img5 :

https://storage.huabio.cn/huabio/productImg/ET7109-38_5.jpg

Pic legend5 :

Immunohistochemical analysis of paraffin-embedded rat spleen tissue with Rabbit anti-FYB antibody (ET7109-38) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET7109-38) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Pic img6 :

https://storage.huabio.cn/huabio/productImg/ET7109-38_6.jpg

Pic legend6 :

Immunocytochemistry analysis of Jurkat cells labeling FYB with Rabbit anti-FYB antibody (ET7109-38) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-FYB antibody (ET7109-38) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (HA601187, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution.

Pic img7 :

https://storage.huabio.cn/huabio/productImg/ET7109-38_7.jpg

Pic legend7 :

Flow cytometric analysis of FYB was done on HepG2 cells. The cells were fixed, permeabilized and stained with FYB antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). After incubation of the primary antibody on room temperature for 1 hour, the cells was stained with a Alexa Fluor™ 488-conjugated goat anti-rabbit IgG Secondary antibody at 1/500 dilution for 30 minutes.

more info or order :

HUABIO product webpage