product summary
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company name :
HUABIO
product type :
antibody
product name :
CBS
catalog :
ET7109-21
quantity :
100μl
price :
385.00 USD
clonality :
monoclonal
host :
domestic rabbit
conjugate :
nonconjugated
clone name :
JE40-99
reactivity :
human
application :
western blot
more info or order :
image
image 1 :
Western blot analysis of CBS on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ET7109-21, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:200,000 dilution was used for 1 hour at room temperature.
Positive control:
Lane 1: 293 cell lysate
Lane 2: Human liver tissue lysate
Predicted band size: 61 kDa
Observed band size: 59 kDa
image 2 :
ICC staining of CBS in 293T cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 10% negative goat serum for 15 minutes at room temperature. Cells were probed with the primary antibody (ET7109-21, 1/200) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 conjugate-Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue).
product information
SKU :
ET7109-21
Target name :
CBS
Species reactivity :
Human
Applications :
WB,IF-Cell
Conjugate :
Non-conjugated
Immunogen :
Recombinant protein within Human CBS aa 360-551 / 551.
Uniprot id :
P35520>SwissProt: P35520 Human
Host :
Rabbit
Clone number :
JE40-99
Isotype :
IgG
Size :
100μl
List Price :
385.00 USD
Storage Buffer :
1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Form :
Liquid
Storage Instruction :
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.
Purity :
Protein A affinity purified.
Product type :
Recombinant Rabbit monoclonal Antibody
Positive control :
293 cell lysate, human liver tissue lysate, 293T.
Molecular wt :
Predicted band size: 61 kDa
Subcellular location :
Cytoplasm. Nucleus.
Concentration :
1 mg/mL.
Recommended dilutions :
WB: 1:500-1:2,000;IF-Cell: 1:50-1:100
more info or order :
company information
HUABIO
Founded in 2007, HUABIO is dedicated to developing high-quality antibodies that advance innovation. We are passionate about the accuracy, efficiency, and consistency of our products. That is why we have invested in new production platforms, like recombinant rabbit monoclonals, alpaca nanobodies, and adopted aggressive QA standards to deliver cutting-edge antibodies with uncompromised quality.
We hope to see you at your next discovery!
We hope to see you at your next discovery!
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