MARK2 | HUABIO ET7109-20 product information

product summary

company name :

HUABIO

product type :

antibody

product name :

MARK2

catalog :

ET7109-20

quantity :

100μl

price :

385.00 USD

clonality :

monoclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

JE40-97

reactivity :

human, mouse, rat

application :

western blot, flow cytometry, immunohistochemistry - paraffin section

more info or order :

HUABIO product webpage

image

image 1 :

Western blot analysis of MARK2 on different lysates with Rabbit anti-MARK2 antibody (ET7109-20) at 1/1,000 dilution. Lane 1: A549-WT cell lysate Lane 2: A549-KD MARK2 cell lysate Lysates/proteins at 10 µg/Lane. Predicted band size: 88 kDa Observed band size: 75/80 kDa Exposure time: 1 minute; ECL: K1802; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET7109-20) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

image 2 :

Western blot analysis of MARK2 on different lysates with Rabbit anti-MARK2 antibody (ET7109-20) at 1/1,000 dilution. Lane 1: Raji cell lysate (10 µg/Lane) Lane 2: U-87 MG cell lysate (10 µg/Lane) Lane 3: Mouse brain tissue lysate (20 µg/Lane) Lane 4: Rat brain tissue lysate (20 µg/Lane) Predicted band size: 88 kDa Observed band size: 75/80 kDa Exposure time: 20 seconds; ECL: K1802; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET7109-20) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

image 3 :

Immunohistochemical analysis of paraffin-embedded human appendix tissue using anti-MARK2 antibody. Counter stained with hematoxylin.

product information

SKU :

ET7109-20

Target name :

MARK2

Species reactivity :

Human,Mouse,Rat

Applications :

WB,IHC-P,FC

Conjugate :

Non-conjugated

Immunogen :

Recombinant protein within Human MARK2 aa 661-789 / 789.

Uniprot id :

Q7KZI7>SwissProt: Q7KZI7 Human;SwissProt: Q05512 Mouse;SwissProt: O08679 Rat

Host :

Rabbit

Clone number :

JE40-97

Isotype :

IgG

Size :

100μl

List Price :

385.00 USD

Storage Buffer :

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Form :

Liquid

Storage Instruction :

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.

Purity :

Protein A affinity purified.

Product type :

Recombinant Rabbit monoclonal Antibody

Positive control :

A549 cell lysate, Raji cell lysate, U-87 MG cell lysate, Mouse brain tissue lysate, Rat brain tissue lysate, human appendix tissue, Raji.

Molecular wt :

Predicted band size: 88 kDa

Subcellular location :

Cell membrane. Cytoplasm. Cytoskeleton.

Concentration :

1 mg/mL.

Recommended dilutions :

WB: 1:500-1:2,000 ;IHC-P: 1:50-1:200 ;FC: 1:1,000

Advanced Validation :

Knockdown (KD)

Pic img4 :

https://storage.huabio.cn/huabio/productImg/ET7109-20_4.jpg

Pic legend4 :

Flow cytometric analysis of Raji cells labeling MARK2. Cells were fixed and permeabilized. Then stained with the primary antibody (ET7109-20, 1/1,000) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody (HA1121) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).

more info or order :

HUABIO product webpage