LysRS | HUABIO ET7109-10 product information

product summary

company name :

HUABIO

product type :

antibody

product name :

LysRS

catalog :

ET7109-10

quantity :

100μl

price :

385.00 USD

clonality :

monoclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

JE40-55

reactivity :

human, mouse, rat

application :

western blot, immunoprecipitation, flow cytometry, immunohistochemistry - paraffin section

more info or order :

HUABIO product webpage

image

image 1 :

Western blot analysis of LysRS on different lysates with Rabbit anti-LysRS antibody (ET7109-10) at 1/2,000 dilution. Lane 1: SiHa cell lysate Lane 2: MCF7 cell lysate Lane 3: NIH/3T3 cell lysate Lane 4: Mouse colon tissue lysate Lane 5: Mouse testis tissue lysate Lane 6: Rat colon tissue lysate Lane 7: Rat testis tissue lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 68 kDa Observed band size: 68 kDa Exposure time: 30 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET7109-10) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

image 2 :

Immunocytochemistry analysis of MCF7 cells labeling LysRS with Rabbit anti-LysRS antibody (ET7109-10) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-LysRS antibody (ET7109-10) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (HA601187, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution.

image 3 :

Immunocytochemistry analysis of NIH/3T3 cells labeling LysRS with Rabbit anti-LysRS antibody (ET7109-10) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-LysRS antibody (ET7109-10) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (HA601187, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution.

product information

SKU :

ET7109-10

Target name :

LysRS

Species reactivity :

Human,Mouse,Rat

Applications :

WB,IHC-P,IF-Cell,IP,FC

Conjugate :

Non-conjugated

Immunogen :

Recombinant protein within Human LysRS aa 460-597 / 597.

Uniprot id :

Q15046>SwissProt: Q15046 Human;SwissProt: Q99MN1 Mouse;SwissProt: Q5XIM7 Rat

Host :

Rabbit

Clone number :

JE40-55

Isotype :

IgG

Size :

100μl

List Price :

385.00 USD

Storage Buffer :

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Form :

Liquid

Storage Instruction :

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.

Purity :

Protein A affinity purified.

Product type :

Recombinant Rabbit monoclonal Antibody

Positive control :

SiHa cell lysate, MCF7 cell lysate, NIH/3T3 cell lysate, Mouse colon tissue lysate, Mouse testis tissue lysate, Rat colon tissue lysate, Rat testis tissue lysate, MCF7, NIH/3T3, human colon tissue, human testis tissue, mouse colon tissue, mouse testis tissue, rat colon tissue, rat testis tissue.

Molecular wt :

Predicted band size: 68 kDa

Subcellular location :

Cytoplasm, cytosol, Nucleus, Cell membrane, Secreted; Mitochondrion.

Concentration :

1 mg/mL.

Recommended dilutions :

WB: 1:2,000 ;IHC-P: 1:200 ;IF-Cell: 1:100 ;IP: 1-2μg/sample ;FC: 1:1,000

Pic img4 :

https://storage.huabio.cn/huabio/productImg/ET7109-10_4.jpg

Pic legend4 :

Immunohistochemical analysis of paraffin-embedded human colon tissue with Rabbit anti-LysRS antibody (ET7109-10) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET7109-10) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Pic img5 :

https://storage.huabio.cn/huabio/productImg/ET7109-10_5.jpg

Pic legend5 :

Immunohistochemical analysis of paraffin-embedded human testis tissue with Rabbit anti-LysRS antibody (ET7109-10) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET7109-10) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Pic img6 :

https://storage.huabio.cn/huabio/productImg/ET7109-10_6.jpg

Pic legend6 :

Immunohistochemical analysis of paraffin-embedded mouse colon tissue with Rabbit anti-LysRS antibody (ET7109-10) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET7109-10) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Pic img7 :

https://storage.huabio.cn/huabio/productImg/ET7109-10_7.jpg

Pic legend7 :

Immunohistochemical analysis of paraffin-embedded mouse testis tissue with Rabbit anti-LysRS antibody (ET7109-10) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET7109-10) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Pic img8 :

https://storage.huabio.cn/huabio/productImg/ET7109-10_8.jpg

Pic legend8 :

Immunohistochemical analysis of paraffin-embedded rat colon tissue with Rabbit anti-LysRS antibody (ET7109-10) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET7109-10) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Pic img9 :

https://storage.huabio.cn/huabio/productImg/ET7109-10_9.jpg

Pic legend9 :

Immunohistochemical analysis of paraffin-embedded rat testis tissue with Rabbit anti-LysRS antibody (ET7109-10) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET7109-10) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Pic img10 :

https://storage.huabio.cn/huabio/productImg/ET7109-10_10.jpg

Pic legend10 :

LysRS was immunoprecipitated from 0.2 mg MCF7 cell lysate with ET7109-10 at 2 µg/10 µl beads. Western blot was performed from the immunoprecipitate using ET7109-10 at 1/2,000 dilution. HRP Conjugated Anti-Rabbit IgG for IP Nano-secondary antibody at 1/5,000 dilution was used for 1 hour at room temperature. Lane 1: MCF7 cell lysate (input) Lane 2: ET7109-10 IP in MCF7 cell lysate Lane 3: Rabbit IgG instead of ET7109-10 in MCF7 cell lysate Blocking/Dilution buffer: 5% NFDM/TBST Exposure time: 13 seconds; ECL: K1801

Pic img11 :

https://storage.huabio.cn/huabio/productImg/ET7109-10_11.jpg

Pic legend11 :

Flow cytometric analysis of MCF7 cells labeling LysRS. Cells were fixed and permeabilized. Then stained with the primary antibody (ET7109-10, 1/1,000) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody (HA1121) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).

Pic img12 :

https://storage.huabio.cn/huabio/productImg/ET7109-10_12.jpg

Pic legend12 :

Flow cytometric analysis of NIH/3T3 cells labeling LysRS. Cells were fixed and permeabilized. Then stained with the primary antibody (ET7109-10, 1/1,000) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody (HA1121) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).

more info or order :

HUABIO product webpage