product summary
Loading...
company name :
HUABIO
product type :
antibody
product name :
VAMP1
catalog :
ET7109-03
quantity :
100μl
price :
385.00 USD
clonality :
monoclonal
host :
domestic rabbit
conjugate :
nonconjugated
clone name :
JG40-19
reactivity :
human, mouse, rat
application :
western blot, immunohistochemistry - paraffin section
more info or order :
image
image 1 :
Western blot analysis of VAMP1 on different lysates with Rabbit anti-VAMP1 antibody (ET7109-03) at 1/500 dilution.
Lane 1: Mouse cerebellum tissue lysate
Lane 2: Rat brain tissue lysate
Lysates/proteins at 20 µg/Lane.
Predicted band size: 13 kDa
Observed band size: 15 kDa
Exposure time: 2 minutes;
15% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET7109-03) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:300,000 dilution was used for 1 hour at room temperature.
image 2 :
ICC staining VAMP1 in 293T cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
image 3 :
ICC staining VAMP1 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
product information
SKU :
ET7109-03
Target name :
VAMP1
Species reactivity :
Human,Mouse,Rat
Applications :
WB,IHC-P,IF-Cell,IF-Tissue
Conjugate :
Non-conjugated
Immunogen :
Recombinant full length protein of human VAMP1.
Uniprot id :
P23763>SwissProt: P23763 Human;SwissProt: Q62442 Mouse;SwissProt: Q63666 Rat
Host :
Rabbit
Clone number :
JG40-19
Isotype :
IgG
Size :
100μl
List Price :
385.00 USD
Storage Buffer :
1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Form :
Liquid
Storage Instruction :
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.
Purity :
Protein A affinity purified.
Product type :
Recombinant Rabbit monoclonal Antibody
Positive control :
Mouse cerebellum tissue lysate, rat brain tissue lysate, 293T, Hela, SH-SY-5Y, human prostate cancer tissue, mouse brain tissue.
Molecular wt :
Predicted band size: 13 kDa
Subcellular location :
Mitochondrion. Synapse. Synaptosome.
Concentration :
1 mg/mL.
Recommended dilutions :
WB: 1:500-1:2,000;IF-Cell: 1:50-1:200;IF-Tissue: 1:50-1:200;IHC-P: 1:50-1:200
Pic img4 :
https://storage.huabio.cn/huabio/productImg/ET7109-03_4.jpg
Pic legend4 :
ICC staining VAMP1 in SH-SY-5Y cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Pic img5 :
https://storage.huabio.cn/huabio/productImg/ET7109-03_5.jpg
Pic legend5 :
Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue using anti-VAMP1 antibody. Counter stained with hematoxylin.
Pic img6 :
https://storage.huabio.cn/huabio/productImg/ET7109-03_6.jpg
Pic legend6 :
Immunohistochemical analysis of paraffin-embedded mouse brain tissue using anti-VAMP1 antibody. Counter stained with hematoxylin.
more info or order :
company information
HUABIO
Founded in 2007, HUABIO is dedicated to developing high-quality antibodies that advance innovation. We are passionate about the accuracy, efficiency, and consistency of our products. That is why we have invested in new production platforms, like recombinant rabbit monoclonals, alpaca nanobodies, and adopted aggressive QA standards to deliver cutting-edge antibodies with uncompromised quality.
We hope to see you at your next discovery!
We hope to see you at your next discovery!
browse more products
questions and comments