product summary
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company name :
HUABIO
product type :
antibody
product name :
ORP150
catalog :
ET7108-84
quantity :
100μl
price :
385.00 USD
clonality :
monoclonal
host :
domestic rabbit
conjugate :
nonconjugated
clone name :
JG39-11
reactivity :
human, mouse, rat
application :
western blot, flow cytometry, immunohistochemistry - paraffin section
more info or order :
image
image 1 :
Western blot analysis of ORP150 on SH-SY5Y cell lysates with Rabbit anti-ORP150 antibody (ET7108-84) at 1/5,000 dilution.
Lysates/proteins at 20 µg/Lane.
Predicted band size: 111 kDa
Observed band size: 150 kDa
Exposure time: 59 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET7108-84) at 1/5,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
image 2 :
Western blot analysis of ORP150 on mouse prostate tissue lysate using anti-ORP150 antibody at 1/5,000 dilution.
image 3 :
Immunohistochemical analysis of paraffin-embedded rat testis tissue using anti-ORP150 antibody. Counter stained with hematoxylin.
product information
SKU :
ET7108-84
Target name :
ORP150
Species reactivity :
Human,Mouse,Rat
Applications :
WB,IHC-P,FC
Conjugate :
Non-conjugated
Immunogen :
Recombinant protein within Human ORP150 aa 1-190 / 999.
Uniprot id :
Q9Y4L1>SwissProt: Q9Y4L1 Human;SwissProt: Q9JKR6 Mouse
Host :
Rabbit
Clone number :
JG39-11
Isotype :
IgG
Size :
100μl
List Price :
385.00 USD
Storage Buffer :
1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Form :
Liquid
Storage Instruction :
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.
Purity :
Protein A affinity purified.
Product type :
Recombinant Rabbit monoclonal Antibody
Positive control :
SH-SY5Y cell lysates, mouse prostate tissue lysates, rat testis tissue, human liver cancer tissue, human small intestine tissue, mouse testis tissue.
Molecular wt :
Predicted band size: 111 kDa
Subcellular location :
Endoplasmic reticulum
Concentration :
1 mg/mL.
Recommended dilutions :
WB: 1:5,000-1:20,000
;IHC-P: 1:50-1:200
;FC: 1:50-1:100
Pic img4 :
https://storage.huabio.cn/huabio/productImg/ET7108-84_4.jpg
Pic legend4 :
Immunohistochemical analysis of paraffin-embedded human liver cancer tissue using anti-ORP150 antibody. Counter stained with hematoxylin.
Pic img5 :
https://storage.huabio.cn/huabio/productImg/ET7108-84_5.jpg
Pic legend5 :
Immunohistochemical analysis of paraffin-embedded human small intestine tissue using anti-ORP150 antibody. Counter stained with hematoxylin.
Pic img6 :
https://storage.huabio.cn/huabio/productImg/ET7108-84_6.jpg
Pic legend6 :
Immunohistochemical analysis of paraffin-embedded mouse testis tissue using anti-ORP150 antibody. Counter stained with hematoxylin.
Pic img7 :
https://storage.huabio.cn/huabio/productImg/ET7108-84_7.jpg
Pic legend7 :
Flow cytometric analysis of HepG2 cells with ORP150 antibody at 1/100 dilution (purple) compared with an unlabelled control (cells without incubation with primary antibody; yellow). Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody.
more info or order :
company information
HUABIO
Founded in 2007, HUABIO is dedicated to developing high-quality antibodies that advance innovation. We are passionate about the accuracy, efficiency, and consistency of our products. That is why we have invested in new production platforms, like recombinant rabbit monoclonals, alpaca nanobodies, and adopted aggressive QA standards to deliver cutting-edge antibodies with uncompromised quality.
We hope to see you at your next discovery!
We hope to see you at your next discovery!
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