product summary
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company name :
HUABIO
product type :
antibody
product name :
p150 CAF1
catalog :
ET7108-83
quantity :
100μl
price :
385.00 USD
clonality :
monoclonal
host :
domestic rabbit
conjugate :
nonconjugated
clone name :
JG38-98
reactivity :
human
application :
western blot, immunoprecipitation, flow cytometry, immunohistochemistry - paraffin section
more info or order :
image
image 1 :
Western blot analysis of p150 CAF1 on K562 (1) and A431 (2) cell lysate using anti-p150 CAF1 antibody at 1/1,000 dilution.
image 2 :
ICC staining p150 CAF1 in 293T cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
image 3 :
ICC staining p150 CAF1 in A431 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
product information
SKU :
ET7108-83
Target name :
p150 CAF1
Species reactivity :
Human
Applications :
WB,IF-Cell,IF-Tissue,IP,IHC-P,FC
Conjugate :
Non-conjugated
Immunogen :
Recombinant protein within Human p150 CAF1 aa 81-195 / 956.
Uniprot id :
Q13111>SwissProt: Q13111 Human
Host :
Rabbit
Clone number :
JG38-98
Isotype :
IgG
Size :
100μl
List Price :
385.00 USD
Storage Buffer :
1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Form :
Liquid
Storage Instruction :
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.
Purity :
Protein A affinity purified.
Product type :
Recombinant Rabbit monoclonal Antibody
Positive control :
K562 cell lysates, A431 cell lysates, 293T, A431, LOVO, human tonsil tissue, human colon tissue, Daudi, human testis tissue, human lymph nodes tissue.
Molecular wt :
107 kDa (Predicted band size)
Subcellular location :
Nucleus.
Concentration :
1 mg/mL.
Recommended dilutions :
WB: 1:500-1:2,000
;IF-Cell: 1:50-1:200
;IF-Tissue: 1:50-1:200
;IHC-P: 1:50-1:200
;IP: 1:10-1:50
;FC: 1:50-1:100
Pic img4 :
https://storage.huabio.cn/huabio/productImg/ET7108-83_4.jpg
Pic legend4 :
ICC staining p150 CAF1 in LOVO cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Pic img5 :
https://storage.huabio.cn/huabio/productImg/ET7108-83_5.jpg
Pic legend5 :
Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-p150 CAF1 antibody. Counter stained with hematoxylin.
Pic img6 :
https://storage.huabio.cn/huabio/productImg/ET7108-83_6.jpg
Pic legend6 :
Immunohistochemical analysis of paraffin-embedded human colon tissue with Rabbit anti-p150 CAF1 antibody (ET7108-83) at 1/200 dilution.
The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET7108-83) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Pic img7 :
https://storage.huabio.cn/huabio/productImg/ET7108-83_7.jpg
Pic legend7 :
Flow cytometric analysis of Daudi cells with p150 CAF1 antibody at 1/100 dilution (purple) compared with an unlabelled control (cells without incubation with primary antibody; yellow). Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody.
Pic img8 :
https://storage.huabio.cn/huabio/productImg/ET7108-83_8.jpg
Pic legend8 :
Immunohistochemical analysis of paraffin-embedded human testis tissue with Rabbit anti-p150 CAF1 antibody (ET7108-83) at 1/200 dilution.
The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET7108-83) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Pic img9 :
https://storage.huabio.cn/huabio/productImg/ET7108-83_9.jpg
Pic legend9 :
Immunohistochemical analysis of paraffin-embedded human lymph nodes tissue with Rabbit anti-p150 CAF1 antibody (ET7108-83) at 1/200 dilution.
The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET7108-83) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
more info or order :
company information
HUABIO
Founded in 2007, HUABIO is dedicated to developing high-quality antibodies that advance innovation. We are passionate about the accuracy, efficiency, and consistency of our products. That is why we have invested in new production platforms, like recombinant rabbit monoclonals, alpaca nanobodies, and adopted aggressive QA standards to deliver cutting-edge antibodies with uncompromised quality.
We hope to see you at your next discovery!
We hope to see you at your next discovery!
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