product summary
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company name :
HUABIO
product type :
antibody
product name :
Carbonic Anhydrase I
catalog :
ET7108-77
quantity :
100μl
price :
385.00 USD
clonality :
monoclonal
host :
domestic rabbit
conjugate :
nonconjugated
clone name :
JG38-71
reactivity :
human, mouse, rat
application :
western blot, immunohistochemistry - paraffin section
more info or order :
image
image 1 :
Western blot analysis of Carbonic Anhydrase I on different lysates with Rabbit anti-Carbonic Anhydrase I antibody (ET7108-77) at 1/1,000 dilution.
Lane 1: TF-1 cell lysate (20 µg/Lane)
Lane 2: K-562 cell lysate (20 µg/Lane)
Lane 3: Zebrafish tissue lysate (40 µg/Lane)
Lane 4: Human lung tissue lysate (40 µg/Lane)
Predicted band size: 29 kDa
Observed band size: 29 kDa
Exposure time: 4 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET7108-77) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
image 2 :
Western blot analysis of Carbonic Anhydrase I on different lysates with Rabbit anti-Carbonic Anhydrase I antibody (ET7108-77) at 1/500 dilution.
Lane 1: Mouse colon tissue lysate
Lane 2: Rat bone marrow tissue lysate
Lysates/proteins at 20 µg/Lane.
Predicted band size: 29 kDa
Observed band size: 29 kDa
Exposure time: 30 seconds;
12% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET7108-77) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:300,000 dilution was used for 1 hour at room temperature.
image 3 :
Immunohistochemical analysis of paraffin-embedded human spleen tissue with Rabbit anti-Carbonic Anhydrase I antibody (ET7108-77) at 1/800 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET7108-77) at 1/800 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
product information
SKU :
ET7108-77
Target name :
Carbonic Anhydrase I
Species reactivity :
Human,Mouse,Rat,Zebrafish
Applications :
WB,IHC-P
Conjugate :
Non-conjugated
Immunogen :
Recombinant protein within Human Carbonic Anhydrase I aa 120-261 / 261.
Uniprot id :
P00915>SwissProt: P00915 Human;SwissProt: P13634 Mouse;SwissProt: B0BNN3 Rat
Host :
Rabbit
Clone number :
JG38-71
Isotype :
IgG
Size :
100μl
List Price :
385.00 USD
Storage Buffer :
1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Form :
Liquid
Storage Instruction :
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.
Purity :
Protein A affinity purified.
Product type :
Recombinant Rabbit monoclonal Antibody
Positive control :
TF-1 cell lysate, K-562 cell lysate, zebrafish tissue lysate, human lung tissue lysate, mouse colon tissue lysate, rat bone marrow tissue lysate, human spleen tissue, human small intestine tissue, mouse spleen tissue.
Molecular wt :
Predicted band size: 29 kDa
Subcellular location :
Cytoplasm
Concentration :
1 mg/mL.
Recommended dilutions :
WB: 1:1,000-1:2,000
;IHC-P: 1:50-1:800
Pic img4 :
https://storage.huabio.cn/huabio/productImg/ET7108-77_4.jpg
Pic legend4 :
Immunohistochemical analysis of paraffin-embedded human small intestine tissue using anti-Carbonic Anhydrase I antibody. Counter stained with hematoxylin.
Pic img5 :
https://storage.huabio.cn/huabio/productImg/ET7108-77_5.jpg
Pic legend5 :
Immunohistochemical analysis of paraffin-embedded mouse spleen tissue using anti-Carbonic Anhydrase I antibody. Counter stained with hematoxylin.
more info or order :
company information
HUABIO
Founded in 2007, HUABIO is dedicated to developing high-quality antibodies that advance innovation. We are passionate about the accuracy, efficiency, and consistency of our products. That is why we have invested in new production platforms, like recombinant rabbit monoclonals, alpaca nanobodies, and adopted aggressive QA standards to deliver cutting-edge antibodies with uncompromised quality.
We hope to see you at your next discovery!
We hope to see you at your next discovery!
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