NOXA2 | HUABIO ET7108-72 product information

product summary

company name :

HUABIO

product type :

antibody

product name :

NOXA2

catalog :

ET7108-72

quantity :

100μl

price :

385.00 USD

clonality :

monoclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

JG86-31

reactivity :

human, mouse, rat

application :

western blot, immunoprecipitation, immunohistochemistry - paraffin section

more info or order :

HUABIO product webpage

image

image 1 :

Western blot analysis of NOXA2 on rat spleen tissue lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ET7108-72, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:40,000 dilution was used for 1 hour at room temperature.

image 2 :

Immunohistochemical analysis of paraffin-embedded rat spleen tissue using anti-NOXA2 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET7108-72, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

image 3 :

Immunohistochemical analysis of paraffin-embedded human spleen tissue using anti-NOXA2 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET7108-72, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

product information

SKU :

ET7108-72

Target name :

NOXA2

Species reactivity :

Human,Mouse,Rat

Applications :

WB,IHC-P,IP

Conjugate :

Non-conjugated

Immunogen :

Recombinant protein within Human NOXA2 aa 180-320 / 526.

Uniprot id :

P19878>SwissProt: P19878 Human;SwissProt: O70145 Mouse;SwissProt: A7E3N2 Rat

Host :

Rabbit

Clone number :

JG86-31

Isotype :

IgG

Size :

100μl

List Price :

385.00 USD

Storage Buffer :

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Form :

Liquid

Storage Instruction :

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.

Purity :

Protein A affinity purified.

Product type :

Recombinant Rabbit monoclonal Antibody

Positive control :

Rat spleen tissue lysates, rat spleen tissue, human spleen tissue, mouse spleen tissue.

Molecular wt :

60/47 kDa

Subcellular location :

Cytoplasm

Concentration :

1 mg/mL.

Recommended dilutions :

WB: 1:500 ;IHC-P: 1:50-1:200 ;IP: 1:10-1:50

Pic img4 :

https://storage.huabio.cn/huabio/productImg/ET7108-72_4.jpg

Pic legend4 :

Immunohistochemical analysis of paraffin-embedded mouse spleen tissue using anti-NOXA2 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET7108-72, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

more info or order :

HUABIO product webpage