product summary
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company name :
HUABIO
product type :
antibody
product name :
Alcohol Dehydrogenase
catalog :
ET7108-64
quantity :
100μl
price :
385.00 USD
clonality :
monoclonal
host :
domestic rabbit
conjugate :
nonconjugated
clone name :
JG82-31
reactivity :
human, mouse, rat
application :
western blot, immunohistochemistry - paraffin section
more info or order :
image
image 1 :
Western blot analysis of Alcohol Dehydrogenase on rat liver tissue lysates with Rabbit anti-Alcohol Dehydrogenase antibody (ET7108-64) at 1/1,000 dilution.
Lysates/proteins at 20 µg/Lane.
Predicted band size:40 kDa
Observed band size: 40 kDa
Exposure time: 2 minutes;
10% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET7108-64) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.
image 2 :
Immunocytochemistry analysis of HepG2 cells labeling Alcohol Dehydrogenase with Rabbit anti-Alcohol Dehydrogenase antibody (ET7108-64) at 1/50 dilution.
Cells were fixed in 4% paraformaldehyde for 10 minutes at 37 ℃, permeabilized with 0.05% Triton X-100 in PBS for 20 minutes, and then blocked with 2% negative goat serum for 30 minutes at room temperature. Cells were then incubated with Rabbit anti-Alcohol Dehydrogenase antibody (ET7108-64) at 1/50 dilution in 2% negative goat serum overnight at 4 ℃. Alexa Fluor®488 conjugate-Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. Nuclear DNA was labelled in blue with DAPI.
image 3 :
Immunohistochemical analysis of paraffin-embedded human liver tissue with Rabbit anti-Alcohol Dehydrogenase antibody (ET7108-64) at 1/50 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET7108-64) at 1/50 dilution for 0.5 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
product information
SKU :
ET7108-64
Target name :
Alcohol Dehydrogenase
Species reactivity :
Human,Mouse,Rat
Applications :
WB,IHC-P,IF-Cell,IF-Tissue
Conjugate :
Non-conjugated
Immunogen :
Recombinant protein within Human Alcohol Dehydrogenase aa 2-107 / 375.
Uniprot id :
P07327>SwissProt: P07327 Human;SwissProt: P00329 Mouse;SwissProt: P06757 Rat
Host :
Rabbit
Clone number :
JG82-31
Isotype :
IgG
Size :
100μl
List Price :
385.00 USD
Storage Buffer :
1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Form :
Liquid
Storage Instruction :
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.
Purity :
Protein A affinity purified.
Product type :
Recombinant Rabbit monoclonal Antibody
Positive control :
Rat liver tissue lysates, HepG2, human liver tissue, mouse liver tissue, rat liver tissue.
Molecular wt :
40 kDa
Subcellular location :
Cytoplasm
Concentration :
1 mg/mL.
Recommended dilutions :
WB: 1:500-1:2,000
;IF-Cell: 1:50-1:100
;IHC-P: 1:50-1:200
Pic img4 :
https://storage.huabio.cn/huabio/productImg/ET7108-64_4.jpg
Pic legend4 :
Immunohistochemical analysis of paraffin-embedded mouse liver tissue with Rabbit anti-Alcohol Dehydrogenase antibody (ET7108-64) at 1/50 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET7108-64) at 1/50 dilution for 0.5 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Pic img5 :
https://storage.huabio.cn/huabio/productImg/ET7108-64_5.jpg
Pic legend5 :
Immunohistochemical analysis of paraffin-embedded rat liver tissue with Rabbit anti-Alcohol Dehydrogenase antibody (ET7108-64) at 1/50 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET7108-64) at 1/50 dilution for 0.5 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
more info or order :
company information
HUABIO
Founded in 2007, HUABIO is dedicated to developing high-quality antibodies that advance innovation. We are passionate about the accuracy, efficiency, and consistency of our products. That is why we have invested in new production platforms, like recombinant rabbit monoclonals, alpaca nanobodies, and adopted aggressive QA standards to deliver cutting-edge antibodies with uncompromised quality.
We hope to see you at your next discovery!
We hope to see you at your next discovery!
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