OS9 | HUABIO ET7108-61 product information

product summary

company name :

HUABIO

product type :

antibody

product name :

OS9

catalog :

ET7108-61

quantity :

100μl

price :

385.00 USD

clonality :

monoclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

JG38-07

reactivity :

human, mouse, rat

application :

western blot, immunoprecipitation, immunohistochemistry - paraffin section

more info or order :

HUABIO product webpage

image

image 1 :

Western blot analysis of OS9 on different lysates with Rabbit anti-OS9 antibody (ET7108-61) at 1/2,000 dilution. Lane 1: HAP1-parental cell lysate Lane 2: HAP1-OS9 KD cell lysate Lysates/proteins at 10 µg/Lane. Predicted band size: 76 kDa Observed band size: 76 kDa Exposure time: 62 seconds; ECL: K1802; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET7108-61) at 1/2,000 dilution was used in K1803 at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

image 2 :

Western blot analysis of OS9 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ET7108-61, 1/1,000) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:200,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: Mouse testis tissue lysate Lane 2: Rat testis tissue lysate

image 3 :

Immunohistochemical analysis of paraffin-embedded rat epididymis tissue using anti-OS9 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes.The tissues were blocked in 1% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET7108-61, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

product information

SKU :

ET7108-61

Target name :

OS9

Species reactivity :

Human,Mouse,Rat

Applications :

WB,IHC-P,IP

Conjugate :

Non-conjugated

Immunogen :

Recombinant protein within Human OS9 aa 561-667 / 667.

Uniprot id :

Q13438>SwissProt: Q13438 Human;SwissProt: Q8K2C7 Mouse;SwissProt: Q5RKH6 Rat

Host :

Rabbit

Clone number :

JG38-07

Isotype :

IgG

Size :

100μl

List Price :

385.00 USD

Storage Buffer :

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Form :

Liquid

Storage Instruction :

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.

Purity :

Protein A affinity purified.

Product type :

Recombinant Rabbit monoclonal Antibody

Positive control :

Mouse testis tissue lysate, rat testis tissue lysate, rat epididymis tissue, human breast carcinoma tissue, human placenta tissue, mouse testis tissue.

Molecular wt :

76 kDa

Subcellular location :

Endoplasmic reticulum

Concentration :

1 mg/mL.

Recommended dilutions :

WB: 1:1,000-1:2,000 ;IHC-P: 1:50-1:200 ;IP: 1:10-1:50

Advanced Validation :

Knockdown (KD)

Pic img4 :

https://storage.huabio.cn/huabio/productImg/ET7108-61_4.jpg

Pic legend4 :

Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using anti-OS9 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes.The tissues were blocked in 1% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET7108-61, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Pic img5 :

https://storage.huabio.cn/huabio/productImg/ET7108-61_5.jpg

Pic legend5 :

Immunohistochemical analysis of paraffin-embedded human placenta tissue using anti-OS9 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes.The tissues were blocked in 1% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET7108-61, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Pic img6 :

https://storage.huabio.cn/huabio/productImg/ET7108-61_6.jpg

Pic legend6 :

Immunohistochemical analysis of paraffin-embedded mouse testis tissue using anti-OS9 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes.The tissues were blocked in 1% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET7108-61, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

more info or order :

HUABIO product webpage