PKC gamma | HUABIO ET7108-42 product information

product summary

company name :

HUABIO

product type :

antibody

product name :

PKC gamma

catalog :

ET7108-42

quantity :

100μl

price :

385.00 USD

clonality :

monoclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

JG69-32

reactivity :

human, mouse, rat

application :

western blot, immunohistochemistry - paraffin section

more info or order :

HUABIO product webpage

image

image 1 :

Western blot analysis of PKC gamma on different lysates with Rabbit anti-PKC gamma antibody (ET7108-42) at 1/1,000 dilution. Lane 1: Mouse brain tissue lysate Lane 2: Mouse cerebellum tissue lysate Lane 3: Rat brain tissue lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 78 kDa Observed band size: 75 kDa Exposure time: 25 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET7108-42) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

image 2 :

Western blot analysis of PKC gamma on A549 cell lysate lysates with Rabbit anti-PKC gamma antibody (ET7108-42) at 1/500 dilution. Lysates/proteins at 10 µg/Lane. Predicted band size: 78 kDa Observed band size: 78 kDa Exposure time: 2 minutes; 10% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET7108-42) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.

image 3 :

Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue with Rabbit anti-PKC gamma antibody (ET7108-42) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET7108-42) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

product information

SKU :

ET7108-42

Target name :

PKC gamma

Species reactivity :

Human,Mouse,Rat

Applications :

WB,IHC-P

Conjugate :

Non-conjugated

Immunogen :

Recombinant protein within Human PKC gamma aa 564-697 / 697.

Uniprot id :

P05129>SwissProt: P05129 Human;SwissProt: P63318 Mouse;SwissProt: P63319 Rat

Host :

Rabbit

Clone number :

JG69-32

Isotype :

IgG

Size :

100μl

List Price :

385.00 USD

Storage Buffer :

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Form :

Liquid

Storage Instruction :

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.

Purity :

Protein A affinity purified.

Product type :

Recombinant Rabbit monoclonal Antibody

Positive control :

Mouse brain tissue lysate, Mouse cerebellum tissue lysate, Rat brain tissue lysate, A549 cell lysates, mouse cerebellum tissue, rat cerebellum tissue.

Molecular wt :

Predicted band size: 78 kDa

Subcellular location :

Cell membrane. Cytoplasm.

Concentration :

1 mg/mL.

Recommended dilutions :

WB: 1:500-1:2,000 ;IHC-P: 1:1,000-1:5,000

Pic img4 :

https://storage.huabio.cn/huabio/productImg/ET7108-42_4.jpg

Pic legend4 :

Immunohistochemical analysis of paraffin-embedded rat cerebellum tissue with Rabbit anti-PKC gamma antibody (ET7108-42) at 1/5,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET7108-42) at 1/5,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

more info or order :

HUABIO product webpage