product summary
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company name :
HUABIO
product type :
antibody
product name :
SOCS2
catalog :
ET7108-40
quantity :
100μl
price :
385.00 USD
clonality :
monoclonal
host :
domestic rabbit
conjugate :
nonconjugated
clone name :
JG36-86
reactivity :
human, mouse, rat
application :
western blot, immunoprecipitation, immunohistochemistry - paraffin section
more info or order :
image
image 1 :
Western blot analysis of SOCS2 on different lysates with Rabbit anti-SOCS2 antibody (ET7108-40) at 1/1,000 dilution.
Lane 1: SH-SY-5Y cell lysate (40 µg/Lane)
Lane 2: NIH/3T3 cell lysate (40 µg/Lane)
Lane 2: PC-12 cell lysate (40 µg/Lane)
Lane 3: Mouse thymus tissue lysate (40 µg/Lane)
Lane 4: Rat thymus tissue lysate (40 µg/Lane)
Predicted band size: 22 kDa
Observed band size: 22 kDa
Exposure time: 3 minutes 53 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET7108-40) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
image 2 :
Immunohistochemical analysis of paraffin-embedded rat epididymis tissue with Rabbit anti-SOCS2 antibody (ET7108-40) at 1/1,000 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET7108-40) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
image 3 :
Immunocytochemistry analysis of LOVO cells labeling SOCS2 with Rabbit anti-SOCS2 antibody (ET7108-40) at 1/50 dilution.
Cells were fixed in 4% paraformaldehyde for 10 minutes at 37 ℃, permeabilized with 0.05% Triton X-100 in PBS for 20 minutes, and then blocked with 2% negative goat serum for 30 minutes at room temperature. Cells were then incubated with Rabbit anti-SOCS2 antibody (ET7108-40) at 1/50 dilution in 2% negative goat serum overnight at 4 ℃.Alexa Fluor®488 Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. Nuclear DNA was labelled in blue with DAPI.
product information
SKU :
ET7108-40
Target name :
SOCS2
Species reactivity :
Human,Mouse,Rat
Applications :
WB,IF-Cell,IF-Tissue,IP,IHC-P
Conjugate :
Non-conjugated
Immunogen :
Recombinant protein within Human SOCS2 aa 1-198 / 198.
Uniprot id :
O14508>SwissProt: O14508 Human;SwissProt: O35717 Mouse;SwissProt: O88582 Rat
Host :
Rabbit
Clone number :
JG36-86
Isotype :
IgG
Size :
100μl
List Price :
385.00 USD
Storage Buffer :
1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Form :
Liquid
Storage Instruction :
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.
Purity :
Protein A affinity purified.
Product type :
Recombinant Rabbit monoclonal Antibody
Positive control :
SH-SY-5Y cell lysate, NIH/3T3 cell lysate, PC-12 cell lysate, mouse thymus tissue lysate, rat thymus tissue lysate, rat epididymis tissue, LOVO, PC-3M, SH-SY-5Y.
Molecular wt :
Predicted band size: 22 kDa
Subcellular location :
Cytosol
Concentration :
1 mg/mL.
Recommended dilutions :
WB: 1:500-1:2,000
;IF-Cell: 1:50-1:200
;IP: 1:10-1:50
;IHC-P: 1:1,000
Pic img4 :
https://storage.huabio.cn/huabio/productImg/ET7108-40_4.jpg
Pic legend4 :
Immunocytochemistry analysis of PC-3M cells labeling SOCS2 with Rabbit anti-SOCS2 antibody (ET7108-40) at 1/50 dilution.
Cells were fixed in 4% paraformaldehyde for 10 minutes at 37 ℃, permeabilized with 0.05% Triton X-100 in PBS for 20 minutes, and then blocked with 2% negative goat serum for 30 minutes at room temperature. Cells were then incubated with Rabbit anti-SOCS2 antibody (ET7108-40) at 1/50 dilution in 2% negative goat serum overnight at 4 ℃.Alexa Fluor®488 Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. Nuclear DNA was labelled in blue with DAPI.
Pic img5 :
https://storage.huabio.cn/huabio/productImg/ET7108-40_5.jpg
Pic legend5 :
Immunocytochemistry analysis of SH-SY-5Y cells labeling SOCS2 with Rabbit anti-SOCS2 antibody (ET7108-40) at 1/50 dilution.
Cells were fixed in 4% paraformaldehyde for 10 minutes at 37 ℃, permeabilized with 0.05% Triton X-100 in PBS for 20 minutes, and then blocked with 2% negative goat serum for 30 minutes at room temperature. Cells were then incubated with Rabbit anti-SOCS2 antibody (ET7108-40) at 1/50 dilution in 2% negative goat serum overnight at 4 ℃.Alexa Fluor®488 Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. Nuclear DNA was labelled in blue with DAPI.
more info or order :
company information
HUABIO
Founded in 2007, HUABIO is dedicated to developing high-quality antibodies that advance innovation. We are passionate about the accuracy, efficiency, and consistency of our products. That is why we have invested in new production platforms, like recombinant rabbit monoclonals, alpaca nanobodies, and adopted aggressive QA standards to deliver cutting-edge antibodies with uncompromised quality.
We hope to see you at your next discovery!
We hope to see you at your next discovery!
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