product summary
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company name :
HUABIO
product type :
antibody
product name :
Nicastrin
catalog :
ET7108-38
quantity :
100μl
price :
385.00 USD
clonality :
monoclonal
host :
domestic rabbit
conjugate :
nonconjugated
clone name :
JG68-33
reactivity :
human, mouse, rat
application :
western blot
more info or order :
image
image 1 :
Western blot analysis of Nicastrin on THP-1 cell lysates with Rabbit anti-Nicastrin antibody (ET7108-38) at 1/1,000 dilution.
Lysates/proteins at 10 µg/Lane.
Predicted band size: 78 kDa
Observed band size: 78-150 kDa
Exposure time: 2 minutes; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET7108-38) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
image 2 :
Western blot analysis of Nicastrin on different lysates with Rabbit anti-Nicastrin antibody (ET7108-38) at 1/1,000 dilution.
Lane 1: NIH/3T3 cell lysate (20 µg/Lane)
Lane 2: Neuro-2a cell lysate (20 µg/Lane)
Lane 3: Mouse brain tissue lysate (40 µg/Lane)
Predicted band size: 78 kDa
Observed band size: 78-150 kDa
Exposure time: 1 minute; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET7108-38) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
image 3 :
Western blot analysis of Nicastrin on different lysates with Rabbit anti-Nicastrin antibody (ET7108-38) at 1/2,000 dilution.
Lane 1: HAP1-parental cell lysate
Lane 2: HAP1-Nicastrin KD cell lysate
Lysates/proteins at 10 µg/Lane.
Predicted band size: 78 kDa
Observed band size: 78-150 kDa
Exposure time: 60 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET7108-38) at 1/2,000 dilution was used in K1803 at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
product information
SKU :
ET7108-38
Target name :
Nicastrin
Species reactivity :
Human,Mouse,Rat
Applications :
WB
Conjugate :
Non-conjugated
Immunogen :
Recomninant protein within Human Nicastrin aa 5-112 / 709.
Uniprot id :
Q92542>SwissProt: Q92542 Human;SwissProt: P57716 Mouse
Host :
Rabbit
Clone number :
JG68-33
Isotype :
IgG
Size :
100μl
List Price :
385.00 USD
Storage Buffer :
1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Form :
Liquid
Storage Instruction :
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.
Purity :
Protein A affinity purified.
Product type :
Recombinant Rabbit monoclonal Antibody
Positive control :
THP-1 cell lysates, NIH/3T3 cell lysate, Neuro-2a cell lysate, Mouse brain tissue lysate.
Molecular wt :
Predicted band size: 78 kDa
Subcellular location :
Membrane, Cytoplasmic vesicle membrane, Melanosome.
Concentration :
1 mg/mL.
Recommended dilutions :
WB: 1:1,000-1:2,000
Advanced Validation :
Knockdown (KD)
more info or order :
company information
HUABIO
Founded in 2007, HUABIO is dedicated to developing high-quality antibodies that advance innovation. We are passionate about the accuracy, efficiency, and consistency of our products. That is why we have invested in new production platforms, like recombinant rabbit monoclonals, alpaca nanobodies, and adopted aggressive QA standards to deliver cutting-edge antibodies with uncompromised quality.
We hope to see you at your next discovery!
We hope to see you at your next discovery!
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