TP53INP1 | HUABIO ET7108-35 product information

product summary

company name :

HUABIO

product type :

antibody

product name :

TP53INP1

catalog :

ET7108-35

quantity :

100μl

price :

385.00 USD

clonality :

monoclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

JG37-64

reactivity :

human, mouse, rat

application :

western blot

more info or order :

HUABIO product webpage

image

image 1 :

Western blot analysis of TP53INP1 on different lysates with Rabbit anti-TP53INP1 antibody (ET7108-35) at 1/1,000 dilution. Lane 1: HepG2 cell lysate Lane 2: RAW264.7 cell lysate Lane 3: C6 cell lysate Lane 4: Human lung tissue lysate Lane 5: Mouse stomach tissue lysate Lane 6: Rat stomach tissue lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 27 kDa Observed band size: 30 kDa Exposure time: 1 minute 50 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET7108-35) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

image 2 :

Immunocytochemistry analysis of HepG2 cells labeling TP53INP1 with Rabbit anti-TP53INP1 antibody (ET7108-35) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 10 minutes at 37 ℃, permeabilized with 0.05% Triton X-100 in PBS for 20 minutes, and then blocked with 2% negative goat serum for 30 minutes at room temperature. Cells were then incubated with Rabbit anti-TP53INP1 antibody (ET7108-35) at 1/50 dilution in 2% negative goat serum overnight at 4 ℃.Alexa Fluor®488 Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution.Nuclear DNA was labelled in blue with DAPI.

product information

SKU :

ET7108-35

Target name :

TP53INP1

Species reactivity :

Human,Mouse,Rat

Applications :

WB,IF-Cell,IF-Tissue

Conjugate :

Non-conjugated

Immunogen :

Recombinant protein within Human p53 DINP1 aa 80-188 / 240.

Uniprot id :

Q96A56>SwissProt: Q96A56 Human;SwissProt: Q9QXE4 Mouse;SwissProt: Q80YE2 Rat

Host :

Rabbit

Clone number :

JG37-64

Isotype :

IgG

Size :

100μl

List Price :

385.00 USD

Storage Buffer :

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Form :

Liquid

Storage Instruction :

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.

Purity :

Protein A affinity purified.

Product type :

Recombinant Rabbit monoclonal Antibody

Positive control :

HepG2 cell lysate, RAW264.7 cell lysate, C6 cell lysate, Human lung tissue lysate, Mouse stomach tissue lysate, Rat stomach tissue lysate, HepG2.

Molecular wt :

Predicted band size: 27 kDa

Subcellular location :

Cytosol. Nucleus.

Concentration :

1 mg/mL.

Recommended dilutions :

WB: 1:1,000 ;IF-Cell: 1:50 ;IF-Tissue: 1:50-1:200

more info or order :

HUABIO product webpage