KAT8 | HUABIO ET7108-23 product information

product summary

company name :

HUABIO

product type :

antibody

product name :

KAT8

catalog :

ET7108-23

quantity :

100μl

price :

385.00 USD

clonality :

monoclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

JG36-05

reactivity :

human, mouse, rat

application :

western blot, flow cytometry, immunohistochemistry - paraffin section

more info or order :

HUABIO product webpage

image

image 1 :

Western blot analysis of KAT8 on different lysates with Rabbit anti-KAT8 antibody (ET7108-23) at 1/1,000 dilution. Lane 1: HEK-293 cell lysate Lane 2: HepG2 cell lysate Lane 3: PC-3M cell lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 52 kDa Observed band size: 55 kDa Exposure time: 3 minutes; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET7108-23) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

image 2 :

Immunocytochemistry analysis of MCF-7 cells labeling KAT8 with Rabbit anti-KAT8 antibody (ET7108-23) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 10 minutes at 37 ℃, permeabilized with 0.05% Triton X-100 in PBS for 20 minutes, and then blocked with 2% negative goat serum for 30 minutes at room temperature. Cells were then incubated with Rabbit anti-KAT8 antibody (ET7108-23) at 1/50 dilution in 2% negative goat serum overnight at 4 ℃.Alexa Fluor®488 Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. Nuclear DNA was labelled in blue with DAPI.

image 3 :

Immunocytochemistry analysis of SH-SY-5Y cells labeling KAT8 with Rabbit anti-KAT8 antibody (ET7108-23) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 10 minutes at 37 ℃, permeabilized with 0.05% Triton X-100 in PBS for 20 minutes, and then blocked with 2% negative goat serum for 30 minutes at room temperature. Cells were then incubated with Rabbit anti-KAT8 antibody (ET7108-23) at 1/50 dilution in 2% negative goat serum overnight at 4 ℃.Alexa Fluor®488 Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. Nuclear DNA was labelled in blue with DAPI.

product information

SKU :

ET7108-23

Target name :

KAT8

Species reactivity :

Human,Rat,Mouse

Applications :

WB,IF-Cell,IF-Tissue,IHC-P,FC

Conjugate :

Non-conjugated

Immunogen :

Recombinant protein within Human KAT8 aa 310-458 / 458.

Uniprot id :

Q9H7Z6>SwissProt: Q9H7Z6 Human;SwissProt: Q9D1P2 Mouse;SwissProt: Q5XI06 Rat

Host :

Rabbit

Clone number :

JG36-05

Isotype :

IgG

Size :

100μl

List Price :

385.00 USD

Storage Buffer :

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Form :

Liquid

Storage Instruction :

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.

Purity :

Protein A affinity purified.

Product type :

Recombinant Rabbit monoclonal Antibody

Positive control :

HEK-293 cell lysate, HepG2 cell lysate, PC-3M cell lysate, MCF-7, SH-SY-5Y, SiHa, human cervix tissue, human cervix cancer tissue, rat cervix tissue, K562.

Molecular wt :

Predicted band size: 52 kDa

Subcellular location :

Chromosome. Nucleus.

Concentration :

1 mg/mL.

Recommended dilutions :

IF-Cell: 1:50-1:200 ;IHC-P: 1:50-1:200 ;FC: 1:50-1:100 ;WB: 1:1,000

Pic img4 :

https://storage.huabio.cn/huabio/productImg/ET7108-23_4.jpg

Pic legend4 :

Immunocytochemistry analysis of SiHa cells labeling KAT8 with Rabbit anti-KAT8 antibody (ET7108-23) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 10 minutes at 37 ℃, permeabilized with 0.05% Triton X-100 in PBS for 20 minutes, and then blocked with 2% negative goat serum for 30 minutes at room temperature. Cells were then incubated with Rabbit anti-KAT8 antibody (ET7108-23) at 1/50 dilution in 2% negative goat serum overnight at 4 ℃.Alexa Fluor®488 Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. Nuclear DNA was labelled in blue with DAPI.

Pic img5 :

https://storage.huabio.cn/huabio/productImg/ET7108-23_5.jpg

Pic legend5 :

Immunohistochemical analysis of paraffin-embedded human cervix tissue with Rabbit anti-KAT8 antibody (ET7108-23) at 1/50 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET7108-23) at 1/50 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Pic img6 :

https://storage.huabio.cn/huabio/productImg/ET7108-23_6.jpg

Pic legend6 :

Immunohistochemical analysis of paraffin-embedded human cervix cancer tissue with Rabbit anti-KAT8 antibody (ET7108-23) at 1/50 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET7108-23) at 1/50 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Pic img7 :

https://storage.huabio.cn/huabio/productImg/ET7108-23_7.jpg

Pic legend7 :

Immunohistochemical analysis of paraffin-embedded rat cervix tissue with Rabbit anti-KAT8 antibody (ET7108-23) at 1/50 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET7108-23) at 1/50 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Pic img8 :

https://storage.huabio.cn/huabio/productImg/ET7108-23_8.jpg

Pic legend8 :

Flow cytometric analysis of K562 cells with KAT8 antibody at 1/100 dilution (yellow) compared with an unlabelled control (cells without incubation with primary antibody; purple).Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody.

more info or order :

HUABIO product webpage