ERp29 | HUABIO ET7108-03 product information

product summary

company name :

HUABIO

product type :

antibody

product name :

ERp29

catalog :

ET7108-03

quantity :

100μl

price :

385.00 USD

clonality :

monoclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

JG34-42

reactivity :

human, mouse, rat

application :

western blot, immunohistochemistry - paraffin section

more info or order :

HUABIO product webpage

image

image 1 :

Western blot analysis of ERp29 on different lysates with Rabbit anti-ERp29 antibody (ET7108-03) at 1/1,000 dilution. Lane 1: HAP1-parental cell lysate (10 µg/Lane) Lane 2: HAP1-ERp29 KD cell lysate (10 µg/Lane) Predicted band size: 29 kDa Observed band size: 29 kDa Exposure time: 12 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET7108-03) at 1/1,000 dilution was used in K1803 at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

image 2 :

Western blot analysis of ERp29 on THP-1 cell lysate using anti-ERp29 antibody at 1/500 dilution.

image 3 :

ICC staining ERp29 in F9 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

product information

SKU :

ET7108-03

Target name :

ERp29

Species reactivity :

Human,Mouse,Rat

Applications :

WB,IHC-P,IF-Cell,IF-Tissue

Conjugate :

Non-conjugated

Immunogen :

Recombinant protein within Human ERp29 aa 1-142 / 261.

Uniprot id :

P30040>SwissProt: P30040 Human;SwissProt: P57759 Mouse;SwissProt: P52555 Rat

Host :

Rabbit

Clone number :

JG34-42

Isotype :

IgG

Size :

100μl

List Price :

385.00 USD

Storage Buffer :

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Form :

Liquid

Storage Instruction :

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.

Purity :

Protein A affinity purified.

Product type :

Recombinant Rabbit monoclonal Antibody

Positive control :

THP-1 cell lysate, F9, PMVEC, SiHa, rat testis tissue, human thyroid gland tissue, human colon cancer tissue.

Molecular wt :

Predicted band size: 29 kDa

Subcellular location :

Endoplasmic reticulum

Concentration :

1 mg/mL.

Recommended dilutions :

WB: 1:1,000 ;IF-Cell: 1:50-1:200 ;IF-Tissue: 1:50-1:200 ;IHC-P: 1:50-1:200

Advanced Validation :

Knockdown (KD)

Pic img4 :

https://storage.huabio.cn/huabio/productImg/ET7108-03_4.jpg

Pic legend4 :

ICC staining ERp29 in PMVEC cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Pic img5 :

https://storage.huabio.cn/huabio/productImg/ET7108-03_5.jpg

Pic legend5 :

ICC staining ERp29 in SiHa cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Pic img6 :

https://storage.huabio.cn/huabio/productImg/ET7108-03_6.jpg

Pic legend6 :

Immunohistochemical analysis of paraffin-embedded rat testis tissue using anti-ERp29 antibody. Counter stained with hematoxylin.

Pic img7 :

https://storage.huabio.cn/huabio/productImg/ET7108-03_7.jpg

Pic legend7 :

Immunohistochemical analysis of paraffin-embedded human thyroid gland tissue using anti-ERp29 antibody. Counter stained with hematoxylin.

Pic img8 :

https://storage.huabio.cn/huabio/productImg/ET7108-03_8.jpg

Pic legend8 :

Immunohistochemical analysis of paraffin-embedded human colon cancer tissue using anti-ERp29 antibody. Counter stained with hematoxylin.

more info or order :

HUABIO product webpage