Glucose 6 phosphate isomerase | HUABIO ET7108-01 product information

product summary

company name :

HUABIO

product type :

antibody

product name :

Glucose 6 phosphate isomerase

catalog :

ET7108-01

quantity :

100μl

price :

385.00 USD

clonality :

monoclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

JG33-86

reactivity :

human

application :

western blot, flow cytometry, immunohistochemistry - paraffin section

more info or order :

HUABIO product webpage

image

image 1 :

Western blot analysis of Glucose 6 phosphate isomerase on different lysates with Rabbit anti-Glucose 6 phosphate isomerase antibody (ET7108-01) at 1/1,000 dilution. Lane 1: HAP1-parental cell lysate Lane 2: HAP1-Glucose 6 phosphate isomerase KD cell lysate Lysates/proteins at 10 µg/Lane. Predicted band size: 63 kDa Observed band size: 54 kDa Exposure time: 20 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET7108-01) at 1/1,000 dilution was used in K1803 at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

image 2 :

Western blot analysis of Glucose 6 phosphate isomerase on A549 cell lysates and HepG2 cell lysates using anti-Glucose 6 phosphate isomerase antibody at 1/1,000 dilution.

image 3 :

ICC staining Glucose 6 phosphate isomerase in MCF-7 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

product information

SKU :

ET7108-01

Target name :

Glucose 6 phosphate isomerase

Species reactivity :

Human

Applications :

WB,IHC-P,FC,IF-Cell,IF-Tissue

Conjugate :

Non-conjugated

Immunogen :

Recombinant protein within Human Glucose 6 phosphate isomerase aa 1-128 / 558.

Uniprot id :

P06744>SwissProt: P06744 Human

Host :

Rabbit

Clone number :

JG33-86

Isotype :

IgG

Size :

100μl

List Price :

385.00 USD

Storage Buffer :

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Form :

Liquid

Storage Instruction :

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.

Purity :

Protein A affinity purified.

Product type :

Recombinant Rabbit monoclonal Antibody

Positive control :

A549 cell lysates, HepG2 cell lysates, LO2, MCF-7, human colon tissue, human prostate tissue, human kidney tissue, A549.

Molecular wt :

Predicted band size: 63 kDa

Subcellular location :

Cytoplasm. Secreted.

Concentration :

1 mg/mL.

Recommended dilutions :

WB: 1:1,000 ;IF-Cell: 1:50-1:200 ;IF-Tissue: 1:50-1:200 ;IHC-P: 1:50-1:200 ;FC: 1:50-1:100

Advanced Validation :

Knockdown (KD)

Pic img4 :

https://storage.huabio.cn/huabio/productImg/ET7108-01_4.jpg

Pic legend4 :

ICC staining Glucose 6 phosphate isomerase in LO2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Pic img5 :

https://storage.huabio.cn/huabio/productImg/ET7108-01_5.jpg

Pic legend5 :

Immunohistochemical analysis of paraffin-embedded human prostate tissue using anti-Glucose 6 phosphate isomerase antibody. Counter stained with hematoxylin.

Pic img6 :

https://storage.huabio.cn/huabio/productImg/ET7108-01_6.jpg

Pic legend6 :

Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-Glucose 6 phosphate isomerase antibody. Counter stained with hematoxylin.

Pic img7 :

https://storage.huabio.cn/huabio/productImg/ET7108-01_7.jpg

Pic legend7 :

Immunohistochemical analysis of paraffin-embedded human colon tissue using anti-Glucose 6 phosphate isomerase antibody. Counter stained with hematoxylin.

Pic img8 :

https://storage.huabio.cn/huabio/productImg/ET7108-01_8.jpg

Pic legend8 :

Flow cytometric analysis of A549 cells with Glucose 6 phosphate isomerase antibody at 1/100 dilution (purple) compared with an unlabelled control (cells without incubation with primary antibody; yellow).Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody.

more info or order :

HUABIO product webpage