eEF1A1 | HUABIO ET7107-75 product information

product summary

company name :

HUABIO

product type :

antibody

product name :

eEF1A1

catalog :

ET7107-75

quantity :

100μl

price :

385.00 USD

clonality :

monoclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

JB44-13

reactivity :

human, mouse, rat

application :

western blot, flow cytometry, immunohistochemistry - paraffin section

more info or order :

HUABIO product webpage

image

image 1 :

Western blot analysis of eEF1A1 on different lysates with Rabbit anti-eEF1A1 antibody (ET7107-75) at 1/2,000 dilution. Lane 1: HeLa cell lysate (10 µg/Lane) Lane 2: MCF7 cell lysate (10 µg/Lane) Lane 3: 293T cell lysate (10 µg/Lane) Lane 4: Jurkat cell lysate (10 µg/Lane) Lane 5: HepG2 cell lysate (10 µg/Lane) Lane 6: A431 cell lysate (10 µg/Lane) Lane 7: THP-1 cell lysate (10 µg/Lane) Lane 8: K-562 cell lysate (10 µg/Lane) Lane 9: C2C12 cell lysate (10 µg/Lane) Lane 10: Neuro-2a cell lysate (10 µg/Lane) Lane 11: NIH/3T3 cell lysate (10 µg/Lane) Lane 12: PC-12 cell lysate (10 µg/Lane) Lane 13: C6 cell lysate (10 µg/Lane) Lane 14: Rat kidney tissue lysate (40 µg/Lane) Predicted band size: 50 kDa Observed band size: 50 kDa Exposure time: 6 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET7107-75) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

image 2 :

Immunohistochemical analysis of paraffin-embedded human breast cancer tissue with Rabbit anti-eEF1A1 antibody (ET7107-75) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET7107-75) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

image 3 :

Immunohistochemical analysis of paraffin-embedded human kidney tissue with Rabbit anti-eEF1A1 antibody (ET7107-75) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET7107-75) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

product information

SKU :

ET7107-75

Target name :

eEF1A1

Species reactivity :

Human,Mouse,Rat

Applications :

WB,IHC-P,FC,IF-Cell,IF-Tissue

Conjugate :

Non-conjugated

Immunogen :

Synthetic peptide within Human eEF1A1 aa 413-462 / 462.

Uniprot id :

P68104>SwissProt: P68104 Human;SwissProt: P10126 Mouse;SwissProt: P62630 Rat

Host :

Rabbit

Clone number :

JB44-13

Isotype :

IgG

Size :

100μl

List Price :

385.00 USD

Storage Buffer :

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Form :

Liquid

Storage Instruction :

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.

Purity :

Protein A affinity purified.

Product type :

Recombinant Rabbit monoclonal Antibody

Positive control :

HeLa cell lysate, MCF7 cell lysate, 293T cell lysate, Jurkat cell lysate, HepG2 cell lysate, A431 cell lysate, THP-1 cell lysate, K-562 cell lysate, C2C12 cell lysate, Neuro-2a cell lysate, NIH/3T3 cell lysate, PC-12 cell lysate, C6 cell lysate, Rat kidney tissue lysate, human breast cancer tissue, human kidney tissue, mouse kidney tissue, rat kidney tissue, HeLa, C2C12, PC-12.

Molecular wt :

Predicted band size: 50 kDa

Subcellular location :

Cell membrane, Cytoplasm, Membrane, Nucleus.

Concentration :

1 mg/mL.

Recommended dilutions :

WB: 1:2,000 ;IF-Cell: 1:100-1:250 ;IF-Tissue: 1:50-1:200 ;IHC-P: 1:200-1:1,000 ;FC: 1:1,000

Pic img4 :

https://storage.huabio.cn/huabio/productImg/ET7107-75_4.jpg

Pic legend4 :

Immunohistochemical analysis of paraffin-embedded mouse kidney tissue with Rabbit anti-eEF1A1 antibody (ET7107-75) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET7107-75) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Pic img5 :

https://storage.huabio.cn/huabio/productImg/ET7107-75_5.jpg

Pic legend5 :

Immunohistochemical analysis of paraffin-embedded rat kidney tissue with Rabbit anti-eEF1A1 antibody (ET7107-75) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET7107-75) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Pic img6 :

https://storage.huabio.cn/huabio/productImg/ET7107-75_6.jpg

Pic legend6 :

Immunocytochemistry analysis of HeLa cells labeling eEF1A1 with Rabbit anti-eEF1A1 antibody (ET7107-75) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-eEF1A1 antibody (ET7107-75) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (M1305-2, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution.

Pic img7 :

https://storage.huabio.cn/huabio/productImg/ET7107-75_7.jpg

Pic legend7 :

Immunocytochemistry analysis of C2C12 cells labeling eEF1A1 with Rabbit anti-eEF1A1 antibody (ET7107-75) at 1/250 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-eEF1A1 antibody (ET7107-75) at 1/250 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (M1305-2, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution.

Pic img8 :

https://storage.huabio.cn/huabio/productImg/ET7107-75_8.jpg

Pic legend8 :

Flow cytometric analysis of C2C12 cells labeling eEF1A1. Cells were fixed and permeabilized. Then stained with the primary antibody (ET7107-75, 1/1,000) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody (HA1121) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).

Pic img9 :

https://storage.huabio.cn/huabio/productImg/ET7107-75_9.jpg

Pic legend9 :

Immunocytochemistry analysis of PC-12 cells labeling eEF1A1 with Rabbit anti-eEF1A1 antibody (ET7107-75) at 1/250 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-eEF1A1 antibody (ET7107-75) at 1/250 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (M1305-2, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution.

Pic img10 :

https://storage.huabio.cn/huabio/productImg/ET7107-75_10.jpg

Pic legend10 :

Flow cytometric analysis of PC-12 cells labeling eEF1A1. Cells were fixed and permeabilized. Then stained with the primary antibody (ET7107-75, 1/1,000) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody (HA1121) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).

more info or order :

HUABIO product webpage