CD27 | HUABIO ET7107-73 product information

product summary

company name :

HUABIO

product type :

antibody

product name :

CD27

catalog :

ET7107-73

quantity :

100μl

price :

385.00 USD

clonality :

monoclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

JB40-98

reactivity :

human, mouse, rat

application :

western blot, immunohistochemistry - paraffin section

more info or order :

HUABIO product webpage

image

image 1 :

Western blot analysis of CD27 on different lysates with Rabbit anti-CD27 antibody (ET7107-73) at 1/2,000 dilution. Lane 1: Raji cell lysate Lane 2: MCF7 cell lysate (negative) Lysates/proteins at 20 µg/Lane. Predicted band size: 29 kDa Observed band size: 50 kDa Exposure time: 1 minute; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET7107-73) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

image 2 :

Immunohistochemical analysis of paraffin-embedded mouse spleen tissue with Rabbit anti-CD27 antibody (ET7107-73) at 1/5,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET7107-73) at 1/5,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

image 3 :

Immunohistochemical analysis of paraffin-embedded rat spleen tissue with Rabbit anti-CD27 antibody (ET7107-73) at 1/5,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET7107-73) at 1/5,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

product information

SKU :

ET7107-73

Target name :

CD27

Species reactivity :

Human,Mouse,Rat

Applications :

WB,IHC-P

Conjugate :

Non-conjugated

Immunogen :

Synthetic peptide corresponding to Human Intracellular domain of CD27 aa 51-100 / 260.

Uniprot id :

P26842>SwissProt: P26842 Human;SwissProt: P41272 Mouse;Entrez Gene: 500318 Rat

Host :

Rabbit

Clone number :

JB40-98

Isotype :

IgG

Size :

100μl

List Price :

385.00 USD

Storage Buffer :

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Form :

Liquid

Storage Instruction :

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.

Purity :

Protein A affinity purified.

Product type :

Recombinant Rabbit monoclonal Antibody

Positive control :

Raji cell lysate, mouse spleen tissue, rat spleen tissue.

Molecular wt :

Predicted band size: 29 kDa

Subcellular location :

Membrane

Concentration :

1 mg/mL.

Recommended dilutions :

WB: 1:2,000 ;IHC-P: 1:5,000

Advanced Validation :

Relative expression (RE)

more info or order :

HUABIO product webpage