CPS1 | HUABIO ET7107-69 product information

product summary

company name :

HUABIO

product type :

antibody

product name :

CPS1

catalog :

ET7107-69

quantity :

100μl

price :

385.00 USD

clonality :

monoclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

JB40-33

reactivity :

human, mouse, rat

application :

western blot, immunoprecipitation, immunohistochemistry - paraffin section

more info or order :

HUABIO product webpage

image

image 1 :

Western blot analysis of CPS1 on different lysates with Rabbit anti-CPS1 antibody (ET7107-69) at 1/1,000 dilution. Lane 1: HepG2 cell lysate (20 µg/Lane) Lane 2: HeLa cell lysate (20 µg/Lane) Lane 3: U-2 OS cell lysate (negative) (20 µg/Lane) Lane 4: Mouse liver tissue lysate (20 µg/Lane) Lane 5: Mouse small intestine tissue lysate (20 µg/Lane) Lane 6: Rat liver tissue lysate (20 µg/Lane) Predicted band size: 165 kDa Observed band size: 150 kDa Exposure time: 10 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET7107-69) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

image 2 :

ICC staining of CPS1 in HepG2 cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 10% negative goat serum for 15 minutes at room temperature. Cells were probed with the primary antibody (ET7107-69, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 conjugate-Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue).

image 3 :

Immunohistochemical analysis of paraffin-embedded human liver tissue with Rabbit anti-CPS1 antibody (ET7107-69) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET7107-69) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

product information

SKU :

ET7107-69

Target name :

CPS1

Species reactivity :

Human,Mouse,Rat

Applications :

WB,IF-Cell,IHC-P,IP

Conjugate :

Non-conjugated

Immunogen :

Recombinant protein within Human CPS1 aa 82-204 / 1,500.

Uniprot id :

P31327>SwissProt: P31327 Human;SwissProt: Q8C196 Mouse;SwissProt: P07756 Rat

Host :

Rabbit

Clone number :

JB40-33

Isotype :

IgG

Size :

100μl

List Price :

385.00 USD

Storage Buffer :

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Form :

Liquid

Storage Instruction :

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.

Purity :

Protein A affinity purified.

Product type :

Recombinant Rabbit monoclonal Antibody

Positive control :

HepG2 cell lysate, HeLa cell lysate, Mouse liver tissue lysate, Mouse small intestine tissue lysate, Rat liver tissue lysate, HepG2, human liver tissue, mouse liver tissue, rat liver tissue.

Molecular wt :

Predicted band size: 165 kDa

Subcellular location :

Mitochondrion, Nucleus.

Concentration :

1 mg/mL.

Recommended dilutions :

WB: 1:500-1:1,000 ;IF-Cell: 1:50-1:200 ;IHC-P: 1:50-1:200 ;IP: Use at an assay dependent concentration.

Advanced Validation :

Relative expression (RE)

Pic img4 :

https://storage.huabio.cn/huabio/productImg/ET7107-69_4.jpg

Pic legend4 :

Immunohistochemical analysis of paraffin-embedded mouse liver tissue with Rabbit anti-CPS1 antibody (ET7107-69) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET7107-69) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Pic img5 :

https://storage.huabio.cn/huabio/productImg/ET7107-69_5.jpg

Pic legend5 :

Immunohistochemical analysis of paraffin-embedded rat liver tissue with Rabbit anti-CPS1 antibody (ET7107-69) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 2 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET7107-69) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

more info or order :

HUABIO product webpage