MCM3 | HUABIO ET7107-66 product information

product summary

company name :

HUABIO

product type :

antibody

product name :

MCM3

catalog :

ET7107-66

quantity :

100μl

price :

385.00 USD

clonality :

monoclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

JB99-35

reactivity :

human, mouse, rat

application :

western blot, flow cytometry, immunohistochemistry - paraffin section

more info or order :

HUABIO product webpage

image

image 1 :

Western blot analysis of MCM3 on mouse thymus tissue (1), NIH-3T3 cell lysates (2) and Hela cell lysates (3) using anti-MCM3 antibody at 1/500 dilution.

image 2 :

ICC staining MCM3 in 293T cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

image 3 :

ICC staining MCM3 in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

product information

SKU :

ET7107-66

Target name :

MCM3

Species reactivity :

Human,Mouse,Rat

Applications :

WB,IF-Cell,IHC-P,FC

Conjugate :

Non-conjugated

Immunogen :

Synthetic peptide within Human MCM3 aa 1-50 / 808.

Uniprot id :

P25205>SwissProt: P25205 Human;SwissProt: P25206 Mouse;SwissProt: D3ZFP4 Rat

Host :

Rabbit

Clone number :

JB99-35

Isotype :

IgG

Size :

100μl

List Price :

385.00 USD

Storage Buffer :

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Form :

Liquid

Storage Instruction :

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.

Purity :

Protein A affinity purified.

Product type :

Recombinant Rabbit monoclonal Antibody

Positive control :

Mouse thymus tissue lysate, NIH-3T3 cell lysates, Hela cell lysates, 293T, HepG2, SH-SY-5Y, human tonsil tissue, human placenta tissue, mouse small intestine tissue, K562.

Molecular wt :

Predicted band size: 91 kDa

Subcellular location :

Chromosome, Nucleus.

Concentration :

1 mg/mL.

Recommended dilutions :

WB: 1:500-1:1,000;IF-Cell: 1:50-1:200;IHC-P: 1:50-1:200;FC: 1:50-1:100

Pic img4 :

https://storage.huabio.cn/huabio/productImg/ET7107-66_4.jpg

Pic legend4 :

ICC staining MCM3 in SH-SY-5Y cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Pic img5 :

https://storage.huabio.cn/huabio/productImg/ET7107-66_5.jpg

Pic legend5 :

Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-MCM3 antibody. Counter stained with hematoxylin.

Pic img6 :

https://storage.huabio.cn/huabio/productImg/ET7107-66_6.jpg

Pic legend6 :

Immunohistochemical analysis of paraffin-embedded human placenta tissue using anti-MCM3 antibody. Counter stained with hematoxylin.

Pic img7 :

https://storage.huabio.cn/huabio/productImg/ET7107-66_7.jpg

Pic legend7 :

Immunohistochemical analysis of paraffin-embedded mouse small intestine tissue using anti-MCM3 antibody. Counter stained with hematoxylin.

Pic img8 :

https://storage.huabio.cn/huabio/productImg/ET7107-66_8.jpg

Pic legend8 :

Flow cytometric analysis of K562 cells with MCM3 antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody.

more info or order :

HUABIO product webpage