Desmoglein 2 | HUABIO ET7107-65 product information

product summary

company name :

HUABIO

product type :

antibody

product name :

Desmoglein 2

catalog :

ET7107-65

quantity :

100μl

price :

385.00 USD

clonality :

monoclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

JB96-37

reactivity :

human, mouse, rat

application :

western blot, flow cytometry, immunohistochemistry - paraffin section

more info or order :

HUABIO product webpage

image

image 1 :

Western blot analysis of Desmoglein 2 on different lysates with Rabbit anti-Desmoglein 2 antibody (ET7107-65) at 1/500 dilution. Lane 1: A431 cell lysate Lane 2: SiHa cell lysate Lysates/proteins at 10 µg/Lane. Predicted band size: 122 kDa Observed band size: 150 kDa Exposure time: 2 minutes; 6% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET7107-65) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:300,000 dilution was used for 1 hour at room temperature.

image 2 :

Immunohistochemical analysis of paraffin-embedded rat heart tissue using anti-Desmoglein 2 antibody. Counter stained with hematoxylin.

image 3 :

Immunohistochemical analysis of paraffin-embedded human colon cancer tissue using anti-Desmoglein 2 antibody. Counter stained with hematoxylin.

product information

SKU :

ET7107-65

Target name :

Desmoglein 2

Species reactivity :

Human,Mouse,Rat

Applications :

WB,IHC-P,FC

Conjugate :

Non-conjugated

Immunogen :

Recombinant protein within Human Desmoglein 2 aa 961-1,118 / 1,118.

Uniprot id :

Q14126>SwissProt: Q14126 Human;SwissProt: O55111 Mouse;SwissProt: F1LYX9 Rat

Host :

Rabbit

Clone number :

JB96-37

Isotype :

IgG

Size :

100μl

List Price :

385.00 USD

Storage Buffer :

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Form :

Liquid

Storage Instruction :

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.

Purity :

Protein A affinity purified.

Product type :

Recombinant Rabbit monoclonal Antibody

Positive control :

A431 cell lysate, SiHa cell lysate, rat heart tissue, human colon cancer tissue, human prostate tissue, human placenta tissue, mouse small intestine tissue, A431.

Molecular wt :

Predicted band size: 122 kDa

Subcellular location :

Cell membrane

Concentration :

1 mg/mL.

Recommended dilutions :

WB: 1:500-1:1,000 ;IHC-P: 1:50-1:200 ;FC: 1:50-1:100

Pic img4 :

https://storage.huabio.cn/huabio/productImg/ET7107-65_4.jpg

Pic legend4 :

Immunohistochemical analysis of paraffin-embedded human prostate tissue using anti-Desmoglein 2 antibody. Counter stained with hematoxylin.

Pic img5 :

https://storage.huabio.cn/huabio/productImg/ET7107-65_5.jpg

Pic legend5 :

Immunohistochemical analysis of paraffin-embedded human placenta tissue using anti-Desmoglein 2 antibody. Counter stained with hematoxylin.

Pic img6 :

https://storage.huabio.cn/huabio/productImg/ET7107-65_6.jpg

Pic legend6 :

Immunohistochemical analysis of paraffin-embedded mouse small intestine tissue using anti-Desmoglein 2 antibody. Counter stained with hematoxylin.

Pic img7 :

https://storage.huabio.cn/huabio/productImg/ET7107-65_7.jpg

Pic legend7 :

Flow cytometric analysis of A431 cells with Desmoglein 2 antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody.

more info or order :

HUABIO product webpage