Proteasome 20S C2 | HUABIO ET7107-59 product information

product summary

company name :

HUABIO

product type :

antibody

product name :

Proteasome 20S C2

catalog :

ET7107-59

quantity :

100μl

price :

385.00 USD

clonality :

monoclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

JB89-30

reactivity :

human, mouse, rat

application :

western blot, immunoprecipitation, flow cytometry, immunohistochemistry - paraffin section

more info or order :

HUABIO product webpage

image

image 1 :

Western blot analysis of Proteasome 20S C2 on different lysates with Rabbit anti-Proteasome 20S C2 antibody (ET7107-59) at 1/500 dilution. Lane 1: PC-3M cell lysate (10 µg/Lane) Lane 2: K562 cell lysate (10 µg/Lane) Lane 3: Rat testis tissue lysate (20 µg/Lane) Lane 4: Mouse testis tissue lysate (20 µg/Lane) Lane 5: Mouse colon tissue lysate (20 µg/Lane) Predicted band size: 30 kDa Observed band size: 30 kDa Exposure time: 30 seconds; 15% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET7107-59) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:300,000 dilution was used for 1 hour at room temperature.

image 2 :

Immunohistochemical analysis of paraffin-embedded human pancreas tissue using anti-Proteasome 20S C2 antibody. Counter stained with hematoxylin.

image 3 :

Immunohistochemical analysis of paraffin-embedded rat testis tissue using anti-Proteasome 20S C2 antibody. Counter stained with hematoxylin.

product information

SKU :

ET7107-59

Target name :

Proteasome 20S C2

Species reactivity :

Human,Mouse,Rat

Applications :

WB,IHC-P,FC,IP

Conjugate :

Non-conjugated

Immunogen :

Synthetic peptide within Human Proteasome 20S C2 aa 214-263 / 263.

Uniprot id :

P25786>SwissProt: P25786 Human;SwissProt: Q9R1P4 Mouse;SwissProt: P18420 Rat

Host :

Rabbit

Clone number :

JB89-30

Isotype :

IgG

Size :

100μl

List Price :

385.00 USD

Storage Buffer :

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Form :

Liquid

Storage Instruction :

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.

Purity :

Protein A affinity purified.

Product type :

Recombinant Rabbit monoclonal Antibody

Positive control :

PC-3M cell lysate, K562 cell lysate, rat testis tissue lysate, mouse testis tissue lysate, mouse colon tissue lysate, human pancreas tissue, rat testis tissue, mouse cerebellum tissue, human liver cancer tissue, K562.

Molecular wt :

Predicted band size: 30 kDa

Subcellular location :

Cytoplasm. Nucleus.

Concentration :

1 mg/mL.

Recommended dilutions :

WB: 1:500-1:2,000 ;IHC-P: 1:50-1:200 ;FC: 1:50-1:100 ;IP: Use at an assay dependent concentration.

Pic img4 :

https://storage.huabio.cn/huabio/productImg/ET7107-59_4.jpg

Pic legend4 :

Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue using anti-Proteasome 20S C2 antibody. Counter stained with hematoxylin.

Pic img5 :

https://storage.huabio.cn/huabio/productImg/ET7107-59_5.jpg

Pic legend5 :

Immunohistochemical analysis of paraffin-embedded human liver cancer tissue using anti-Proteasome 20S C2 antibody. Counter stained with hematoxylin.

Pic img6 :

https://storage.huabio.cn/huabio/productImg/ET7107-59_6.jpg

Pic legend6 :

Flow cytometric analysis of K562 cells with Proteasome 20S C2 antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.

more info or order :

HUABIO product webpage