Securin | HUABIO ET7107-50 product information

product summary

company name :

HUABIO

product type :

antibody

product name :

Securin

catalog :

ET7107-50

quantity :

100μl

price :

385.00 USD

clonality :

monoclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

JB37-37

reactivity :

human

application :

western blot, immunoprecipitation, flow cytometry, immunohistochemistry - paraffin section

more info or order :

HUABIO product webpage

image

image 1 :

Western blot analysis of Securin on SiHa cell lysate using anti-Securin antibody at 1/500 dilution.

image 2 :

ICC staining Securin in A431 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

image 3 :

ICC staining Securin in HUVEC cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

product information

SKU :

ET7107-50

Target name :

Securin

Species reactivity :

Human

Applications :

WB,IF-Cell,IF-Tissue,IHC-P,FC,IP

Conjugate :

Non-conjugated

Immunogen :

Recombinant protein within C-terminal Human Securin .

Uniprot id :

O95997>SwissProt: O95997 Human;SwissProt: Q6IAL9 Human

Host :

Rabbit

Clone number :

JB37-37

Isotype :

IgG

Size :

100μl

List Price :

385.00 USD

Storage Buffer :

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Form :

Liquid

Storage Instruction :

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.

Purity :

Protein A affinity purified.

Product type :

Recombinant Rabbit monoclonal Antibody

Positive control :

SiHa cell lysate, A431, HUVEC, PC-3M, human tonsil tissue, human colon cancer tissue, human testis tissue, human lymph nodes tissue.

Molecular wt :

Predicted band size: 22 kDa

Subcellular location :

Cytoplasm. Nucleus.

Concentration :

1 mg/mL.

Recommended dilutions :

WB: 1:500 ;IF-Cell: 1:50-1:200 ;IHC-P: 1:50-1:200 ;FC: 1:50-1:100 ;IP: Use at an assay dependent concentration.

Pic img4 :

https://storage.huabio.cn/huabio/productImg/ET7107-50_4.jpg

Pic legend4 :

ICC staining Securin in PC-3M cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Pic img5 :

https://storage.huabio.cn/huabio/productImg/ET7107-50_5.jpg

Pic legend5 :

Immunohistochemical analysis of paraffin-embedded human tonsil tissue with Rabbit anti-Securin antibody (ET7107-50) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET7107-50) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Pic img6 :

https://storage.huabio.cn/huabio/productImg/ET7107-50_6.jpg

Pic legend6 :

Immunohistochemical analysis of paraffin-embedded human colon cancer tissue using anti-Securin antibody. Counter stained with hematoxylin.

Pic img7 :

https://storage.huabio.cn/huabio/productImg/ET7107-50_7.jpg

Pic legend7 :

Flow cytometric analysis of A431 cells with Securin antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody.

Pic img8 :

https://storage.huabio.cn/huabio/productImg/ET7107-50_8.jpg

Pic legend8 :

Immunohistochemical analysis of paraffin-embedded human testis tissue with Rabbit anti-Securin antibody (ET7107-50) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET7107-50) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Pic img9 :

https://storage.huabio.cn/huabio/productImg/ET7107-50_9.jpg

Pic legend9 :

Immunohistochemical analysis of paraffin-embedded human lymph nodes tissue with Rabbit anti-Securin antibody (ET7107-50) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET7107-50) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

more info or order :

HUABIO product webpage