antibody

NPHS2

ET7107-34

100μl

385.00 USD

monoclonal

domestic rabbit

nonconjugated

JB51-33

human, mouse, rat

western blot, flow cytometry, immunohistochemistry - paraffin section

NPHS2

Human,Mouse,Rat

WB,IHC-P,FC,IF-Tissue,mIHC

Non-conjugated

Synthetic peptide within Human NPHS2 aa 334-383 / 383.

Q9NP85>SwissProt: Q9NP85 Human;SwissProt: Q91X05 Mouse;SwissProt: Q8K4G9 Rat

Rabbit

JB51-33

IgG

100μl

385.00 USD

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Liquid

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.

Protein A affinity purified.

Recombinant Rabbit monoclonal Antibody

Rat kidney tissue lysates, human kidney tissue lysate, mouse kidney tissue lysate, human kidney tissue, mouse kidney tissue, rat kidney tissue, 293T.

Predicted band size: 42 kDa

Cell membrane. Endoplasmic reticulum.

1 mg/mL.

WB: 1:500-1:1,000 ;IHC-P: 1:50-1:200 ;FC: 1:50-1:100 ;IF-Tissue: 1:100 ;mIHC: 1:1,000

https://storage.huabio.cn/huabio/productImg/ET7107-34_4.jpg

Immunohistochemical analysis of paraffin-embedded mouse kidney tissue using anti-NPHS2 antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET7107-34, 1/200) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

https://storage.huabio.cn/huabio/productImg/ET7107-34_5.jpg

Immunohistochemical analysis of paraffin-embedded rat kidney tissue using anti-NPHS2 antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET7107-34, 1/200) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

https://storage.huabio.cn/huabio/productImg/ET7107-34_6.jpg

Immunofluorescence analysis of paraffin-embedded human kidney tissue labeling NPHS2 (ET7107-34) and Vimentin (EM0401). The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS. And then probed with the primary antibodies NPHS2 (ET7107-34, red) at 1/100 dilution and Vimentin (EM0401, green) at 1/400 dilution overnight at 4 ℃, washed with PBS. iFluor™ 594 conjugate-Goat anti-Rabbit IgG (HA1122) and iFluor™ 488 conjugate-Goat anti-Mouse IgG (HA1125) were used as the secondary antibodies at 1/1,000 dilution. DAPI was used as nuclear counterstain.

https://storage.huabio.cn/huabio/productImg/ET7107-34_7.jpg

Fluorescence multiplex immunohistochemical analysis of mouse kidney (Formalin/PFA-fixed paraffin-embedded sections). Panel A: the merged image of anti-NPHS2 (ET7107-34, Red), anti-AQP1 (ET1703-34, Green), anti-Laminin beta 1 (ET1703-14, Cyan) and anti-aSMA (ET1607-53, Magenta) on kidney. HRP Conjugated UltraPolymer Goat Polyclonal Antibody HA1119/HA1120 was used as a secondary antibody. The immunostaining was performed with the Sequential Immuno-staining Kit (IRISKit™MH010101, www.luminiris.cn). The section was incubated in four rounds of staining: in the order of ET7107-34 (1/1,000 dilution), ET1703-34 (1/5,000 dilution), ET1703-14 (1/1,000 dilution) and ET1607-53 (1/10,000 dilution) for 20 mins at room temperature. Each round was followed by a separate fluorescent tyramide signal amplification system. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 30 mins at 95℃. DAPI (blue) was used as a nuclear counter stain. Image acquisition was performed with Olympus VS200 Slide Scanner.

https://storage.huabio.cn/huabio/productImg/ET7107-34_8.jpg

Fluorescence multiplex immunohistochemical analysis of mouse kidney (Formalin/PFA-fixed paraffin-embedded sections). Panel A: the merged image of anti-Laminin beta 1 (ET1703-14, Yellow), anti-NPHS2 (ET7107-34, Red) and anti-SLC12A1/NKCC2 (HA721906, Violet) on kidney. HRP Conjugated UltraPolymer Goat Polyclonal Antibody HA1119/HA1120 was used as a secondary antibody. The immunostaining was performed with the Sequential Immuno-staining Kit (IRISKit™MH010101, www.luminiris.cn). The section was incubated in three rounds of staining: in the order of ET1703-14 (1/1,000 dilution), ET7107-34 (1/1,000 dilution) and HA721906 (1/3,000 dilution) for 20 mins at room temperature. Each round was followed by a separate fluorescent tyramide signal amplification system. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 30 mins at 95℃. DAPI (blue) was used as a nuclear counter stain. Image acquisition was performed with Zeiss Observer 7 Inverted Fluorescence Microscope.

https://storage.huabio.cn/huabio/productImg/ET7107-34_9.jpg

Flow cytometric analysis of NPHS2 was done on 293T cells. The cells were fixed, permeabilized and stained with the primary antibody (ET7107-34, 1/50) (red). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated Goat anti-Rabbit IgG Secondary antibody at 1/1,000 dilution for 30 minutes.Unlabelled sample was used as a control (cells without incubation with primary antibody; black).