Anti-Alpha Synuclein Antibody [JB42-33] | HUABIO ET7107-31TR product information

product summary

company name :

HUABIO

product type :

antibody

product name :

Anti-Alpha Synuclein Antibody [JB42-33]

catalog :

ET7107-31TR

quantity :

20 uL

price :

99 USD

clonality :

monoclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

JB42-33

reactivity :

human, mouse

application :

western blot, immunoprecipitation, flow cytometry, immunohistochemistry - paraffin section

more info or order :

HUABIO product webpage

product information

SKU :

ET7107-31TR

Target name :

Anti-Alpha Synuclein Antibody [JB42-33]

Species reactivity :

Human,Mouse

Applications :

WB,IF-Cell,IF-Tissue,IHC-P,IP,FC

Conjugate :

Non-conjugated

Immunogen :

Recombinant full length protein of human Alpha Synuclein.

Uniprot id :

SwissProt: P37840 Human;SwissProt: O55042 Mouse

Host :

Rabbit

Clone number :

JB42-33

Isotype :

IgG

Size :

20 uL

List Price :

99 USD

Storage Buffer :

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Form :

Liquid

Storage Instruction :

Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.

Purity :

Protein A affinity purified.

Product type :

Recombinant Rabbit monoclonal Antibody

Positive control :

Human brain tissue lysates, Hela, mouse brain tissue, SH-SY5Y.

Molecular wt :

Predicted band size: 14 kDa

Subcellular location :

Secreted, nucleus, cytoplasm, membrane, synapse.

Concentration :

1 mg/mL.

Recommended dilutions :

WB: 1:500-1:2,000;IF-Cell: 1:50-1:200;IHC-P: 1:50-1:200;FC: 1:50-1:100;IP: Use at an assay dependent concentration.

Pic img4 :

ET7107-31_2.jpg

Pic img5 :

ICC staining of Alpha Synuclein in Hela cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (ET7107-31, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue).

Pic legend5 :

ET7107-31_3.jpg

Pic legend6 :

Immunohistochemical analysis of paraffin-embedded mouse brain tissue using anti-Alpha Synuclein antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET7107-31, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Pic img7 :

ET7107-31_4.jpg

Pic img8 :

Flow cytometric analysis of Alpha Synuclein was done on SH-SY5Y cells. The cells were fixed, permeabilized and stained with the primary antibody (ET7107-31, 1/50) (red). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated Goat anti-Rabbit IgG Secondary antibody at 1/1000 dilution for 30 minutes.Unlabelled sample was used as a control (cells without incubation with primary antibody; black).

more info or order :

HUABIO product webpage