GRASP65 | HUABIO ET7107-13 product information

product summary

company name :

HUABIO

product type :

antibody

product name :

GRASP65

catalog :

ET7107-13

quantity :

100μl

price :

385.00 USD

clonality :

monoclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

JB36-34

reactivity :

human

application :

western blot, immunoprecipitation, flow cytometry, immunohistochemistry - paraffin section

more info or order :

HUABIO product webpage

image

image 1 :

Western blot analysis of GRASP65 on different lysates with Rabbit anti-GRASP65 antibody (ET7107-13) at 1/1,000 dilution. Lane 1: SK-Br-3 cell lysate Lane 2: HepG2 cell lysate Lane 3: 293T cell lysate Lane 4: HeLa cell lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 46 kDa Observed band size: 70 kDa Exposure time: 25 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET7107-13) at 1/1,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

image 2 :

Immunocytochemistry analysis of HepG2 cells labeling GRASP65 with Rabbit anti-GRASP65 antibody (ET7107-13) at 1/200 dilution. Cells were fixed in 4% paraformaldehyde for 10 minutes at 37 ℃, permeabilized with 0.05% Triton X-100 in PBS for 20 minutes, and then blocked with 2% negative goat serum for 30 minutes at room temperature. Cells were then incubated with Rabbit anti-GRASP65 antibody (ET7107-13) at 1/200 dilution in 2% negative goat serum overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (M1305-2, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution.

image 3 :

Immunohistochemical analysis of paraffin-embedded human uterus tissue with Rabbit anti-GRASP65 antibody (ET7107-13) at 1/100 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET7107-13) at 1/100 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

product information

SKU :

ET7107-13

Target name :

GRASP65

Species reactivity :

Human

Applications :

WB,IF-Cell,IF-Tissue,IHC-P,FC,IP

Conjugate :

Non-conjugated

Immunogen :

Synthetic peptide within Human GRASP65 aa 396-440 / 440.

Uniprot id :

Q9BQQ3>SwissProt: Q9BQQ3 Human

Host :

Rabbit

Clone number :

JB36-34

Isotype :

IgG

Size :

100μl

List Price :

385.00 USD

Storage Buffer :

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Form :

Liquid

Storage Instruction :

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.

Purity :

Protein A affinity purified.

Product type :

Recombinant Rabbit monoclonal Antibody

Positive control :

SK-Br-3 cell lysate, HepG2 cell lysate, 293T cell lysate, HeLa cell lysate, HepG2, human uterus tissue, human colon carcinoma tissue, MCF-7.

Molecular wt :

Predicted band size: 46 kDa

Subcellular location :

Golgi apparatus

Concentration :

1 mg/mL.

Recommended dilutions :

WB: 1:1,000 ;IF-Cell: 1:50-1:200 ;IHC-P: 1:50-1:200 ;FC: 1:50-1:100 ;IP: Use at an assay dependent concentration.

Pic img4 :

https://storage.huabio.cn/huabio/productImg/ET7107-13_4.jpg

Pic legend4 :

Immunohistochemical analysis of paraffin-embedded human colon carcinoma tissue using anti-GRASP65 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes.The tissues were blocked in 1% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET7107-13, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Pic img5 :

https://storage.huabio.cn/huabio/productImg/ET7107-13_5.jpg

Pic legend5 :

Flow cytometric analysis of GRASP65 was done on MCF-7 cells. The cells were fixed, permeabilized and stained with the primary antibody (ET7107-13, 1/50) (red). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor®488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1,000 dilution for 30 minutes.Unlabelled sample was used as a control (cells without incubation with primary antibody; black).

more info or order :

HUABIO product webpage