product summary
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company name :
HUABIO
product type :
antibody
product name :
EB3
catalog :
ET7106-99
quantity :
100μl
price :
385.00 USD
clonality :
monoclonal
host :
domestic rabbit
conjugate :
nonconjugated
clone name :
JU38-30
reactivity :
human, mouse, rat
application :
western blot, immunoprecipitation
more info or order :
image
image 1 :
Western blot analysis of EB3 on different lysates with Rabbit anti-EB3 antibody (ET7106-99) at 1/2,000 dilution.
Lane 1: SH-SY5Y cell lysate (20 µg/Lane)
Lane 2: U-87 MG cell lysate (20 µg/Lane)
Lane 3: MG-63 cell lysate (20 µg/Lane)
Lane 4: Mouse brain tissue lysate (20 µg/Lane)
Lane 5: Rat brain tissue lysate (20 µg/Lane)
Predicted band size: 32 kDa
Observed band size: 35 kDa
Exposure time: Lane 1-3: 30 seconds; Lane 4-5: 8 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET7106-99) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
image 2 :
EB3 was immunoprecipitated from 0.2 mg U-87 MG cell lysate with ET7106-99 at 2 µg/10 µl beads. Western blot was performed from the immunoprecipitate using ET7106-99 at 1/2,000 dilution. HRP Conjugated Anti-Rabbit IgG for IP Nano-secondary antibody at 1/5,000 dilution was used for 1 hour at room temperature.
Lane 1: U-87 MG cell lysate (input)
Lane 2: ET7106-99 IP in U-87 MG cell lysate
Lane 3: Rabbit IgG instead of ET7106-99 in U-87 MG cell lysate
Blocking/Dilution buffer: 5% NFDM/TBST
Exposure time: 1 minute 7 seconds; ECL: K1801
product information
SKU :
ET7106-99
Target name :
EB3
Species reactivity :
Human,Mouse,Rat
Applications :
WB,IP
Conjugate :
Non-conjugated
Immunogen :
Recombinant protein within Human EB3 aa 165-281 / 281.
Uniprot id :
Q9UPY8>SwissProt: Q9UPY8 Human;SwissProt: Q6PER3 Mouse;SwissProt: Q5XIT1 Rat
Host :
Rabbit
Clone number :
JU38-30
Isotype :
IgG
Size :
100μl
List Price :
385.00 USD
Storage Buffer :
1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Form :
Liquid
Storage Instruction :
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.
Purity :
Protein A affinity purified.
Product type :
Recombinant Rabbit monoclonal Antibody
Positive control :
SH-SY5Y cell lysate, U-87 MG cell lysate, MG-63 cell lysate, Mouse brain tissue lysate, Rat brain tissue lysate.
Molecular wt :
Predicted band size: 32 kDa
Subcellular location :
Cytoplasm, cytoskeleton.
Concentration :
1 mg/mL.
Recommended dilutions :
WB: 1:2,000
;IP: 1-2μg/sample
more info or order :
company information
HUABIO
Founded in 2007, HUABIO is dedicated to developing high-quality antibodies that advance innovation. We are passionate about the accuracy, efficiency, and consistency of our products. That is why we have invested in new production platforms, like recombinant rabbit monoclonals, alpaca nanobodies, and adopted aggressive QA standards to deliver cutting-edge antibodies with uncompromised quality.
We hope to see you at your next discovery!
We hope to see you at your next discovery!
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