product summary
Loading...
company name :
HUABIO
product type :
antibody
product name :
PCAF
catalog :
ET7106-79
quantity :
100μl
price :
385.00 USD
clonality :
monoclonal
host :
domestic rabbit
conjugate :
nonconjugated
clone name :
JU88-63
reactivity :
human, mouse, rat
application :
western blot, immunoprecipitation, flow cytometry
more info or order :
image
image 1 :
Western blot analysis of PCAF on HeLa cell lysates with Rabbit anti-PCAF antibody (ET7106-79) at 1/2,000 dilution.
Lysates/proteins at 20 µg/Lane.
Predicted band size: 93 kDa
Observed band size: 93 kDa
Exposure time: 5 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET7106-79) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
image 2 :
Flow cytometric analysis of Hela cells with PCAF antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody.
product information
SKU :
ET7106-79
Target name :
PCAF
Species reactivity :
Human,Mouse,Rat
Applications :
WB,IP,FC
Conjugate :
Non-conjugated
Immunogen :
Synthetic peptide within Human PCAF aa 121-211 .
Uniprot id :
Q92831>SwissProt: Q92831 Human;SwissProt: Q568Z2 Rat
Host :
Rabbit
Clone number :
JU88-63
Isotype :
IgG
Size :
100μl
List Price :
385.00 USD
Storage Buffer :
1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Form :
Liquid
Storage Instruction :
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.
Purity :
Protein A affinity purified.
Product type :
Recombinant Rabbit monoclonal Antibody
Positive control :
HeLa cell lysates, Hela.
Molecular wt :
Predicted band size: 93 kDa
Subcellular location :
Nucleus.
Concentration :
1 mg/mL.
Recommended dilutions :
WB: 1:2,000
;FC: 1:50-1:100
;IP: 1:10-1:50
more info or order :
company information
HUABIO
Founded in 2007, HUABIO is dedicated to developing high-quality antibodies that advance innovation. We are passionate about the accuracy, efficiency, and consistency of our products. That is why we have invested in new production platforms, like recombinant rabbit monoclonals, alpaca nanobodies, and adopted aggressive QA standards to deliver cutting-edge antibodies with uncompromised quality.
We hope to see you at your next discovery!
We hope to see you at your next discovery!
browse more products
questions and comments