ALDH1L1 | HUABIO ET7106-64 product information

product summary

company name :

HUABIO

product type :

antibody

product name :

ALDH1L1

catalog :

ET7106-64

quantity :

100μl

price :

385.00 USD

clonality :

monoclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

JU53-54

reactivity :

human, mouse, rat

application :

western blot, immunohistochemistry - paraffin section, immunohistochemistry - frozen section

more info or order :

HUABIO product webpage

image

image 1 :

Application: IHC-Fr Species: Mouse Site: Cerebral cortex Sample: Frozen section Antibody concentration: 1:500 Antigen retrieval: Not required

image 2 :

Application: IHC-Fr Species: Rat Site: Cerebral cortex Sample: Frozen section Antibody concentration: 1:500 Antigen retrieval: Not required

image 3 :

Application: IF-tissue Species: Mouse Site: Cerebral cortex Sample: Paraffin-embedded section Antibody concentration: 1:500

product information

SKU :

ET7106-64

Target name :

ALDH1L1

Species reactivity :

Human,Mouse,Rat,Cynomolgus monkey,Pig

Applications :

WB,IHC-P,IF-Tissue,IHC-Fr

Conjugate :

Non-conjugated

Immunogen :

Recombinant protein within Human ALDH1L1 aa 155-375 .

Uniprot id :

O75891>SwissProt: O75891 Human;SwissProt: Q8R0Y6 Mouse;SwissProt: P28037 Rat

Host :

Rabbit

Clone number :

JU53-54

Isotype :

IgG

Size :

100μl

List Price :

385.00 USD

Storage Buffer :

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Form :

Liquid

Storage Instruction :

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.

Purity :

Protein A affinity purified.

Product type :

Recombinant Rabbit monoclonal Antibody

Positive control :

Human brain tissue, mouse brain tissue, rat hippocampus tissue, human kidney tissue, mouse kidney tissue, rat kidney tissue, human liver tissue, mouse liver tissue, rat liver tissue, Mouse brain tissue lysate, Mouse liver tissue lysate, Mouse kidney tissue lysate, Rat brain tissue lysate, Rat liver tissue lysate, Rat kidney tissue lysate.

Molecular wt :

Predicted band size: 99 kDa

Subcellular location :

Cytoplasm

Concentration :

1 mg/mL.

Recommended dilutions :

WB: 1:5,000-1:10,000 ;IHC-P: 1:500-1:2,000 ;IF-Tissue: 1:500 ;IHC-Fr: 1:500-1:1,000

Pic img4 :

https://storage.huabio.cn/huabio/productImg/ET7106-64_4.jpg

Pic legend4 :

Application: IF-tissue Species: Rat Site: Cerebral cortex Sample: Paraffin-embedded section Antibody concentration: 1:500

Pic img5 :

https://storage.huabio.cn/huabio/productImg/ET7106-64_5.jpg

Pic legend5 :

Immunohistochemical analysis of paraffin-embedded human brain tissue with Rabbit anti-ALDH1L1 antibody (ET7106-64) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET7106-64) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Pic img6 :

https://storage.huabio.cn/huabio/productImg/ET7106-64_6.jpg

Pic legend6 :

Immunohistochemical analysis of paraffin-embedded mouse brain tissue with Rabbit anti-ALDH1L1 antibody (ET7106-64) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET7106-64) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Pic img7 :

https://storage.huabio.cn/huabio/productImg/ET7106-64_7.jpg

Pic legend7 :

Immunohistochemical analysis of paraffin-embedded rat hippocampus tissue with Rabbit anti-ALDH1L1 antibody (ET7106-64) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET7106-64) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Pic img8 :

https://storage.huabio.cn/huabio/productImg/ET7106-64_8.jpg

Pic legend8 :

Immunohistochemical analysis of paraffin-embedded human kidney tissue with Rabbit anti-ALDH1L1 antibody (ET7106-64) at 1/500 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET7106-64) at 1/500 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Pic img9 :

https://storage.huabio.cn/huabio/productImg/ET7106-64_9.jpg

Pic legend9 :

Immunohistochemical analysis of paraffin-embedded mouse kidney tissue with Rabbit anti-ALDH1L1 antibody (ET7106-64) at 1/2,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET7106-64) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Pic img10 :

https://storage.huabio.cn/huabio/productImg/ET7106-64_10.jpg

Pic legend10 :

Immunohistochemical analysis of paraffin-embedded rat kidney tissue with Rabbit anti-ALDH1L1 antibody (ET7106-64) at 1/2,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET7106-64) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Pic img11 :

https://storage.huabio.cn/huabio/productImg/ET7106-64_11.jpg

Pic legend11 :

Immunohistochemical analysis of paraffin-embedded human liver tissue with Rabbit anti-ALDH1L1 antibody (ET7106-64) at 1/2,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET7106-64) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Pic img12 :

https://storage.huabio.cn/huabio/productImg/ET7106-64_12.jpg

Pic legend12 :

Immunohistochemical analysis of paraffin-embedded mouse liver tissue with Rabbit anti-ALDH1L1 antibody (ET7106-64) at 1/2,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET7106-64) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Pic img13 :

https://storage.huabio.cn/huabio/productImg/ET7106-64_13.jpg

Pic legend13 :

Immunohistochemical analysis of paraffin-embedded rat liver tissue with Rabbit anti-ALDH1L1 antibody (ET7106-64) at 1/2,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET7106-64) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Pic img14 :

https://storage.huabio.cn/huabio/productImg/ET7106-64_14.jpg

Pic legend14 :

Western blot analysis of ALDH1L1 on different lysates with Rabbit anti-ALDH1L1 antibody (ET7106-64) at 1/5,000 dilution. Lane 1: Mouse brain tissue lysate Lane 2: Mouse liver tissue lysate Lane 3: Mouse kidney tissue lysate Lane 4: Rat brain tissue lysate Lane 5: Rat liver tissue lysate Lane 6: Rat kidney tissue lysate Lysates/proteins at 40 µg/Lane. Predicted band size: 99 kDa Observed band size: 99 kDa Exposure time: 10 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET7106-64) at 1/5,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

more info or order :

HUABIO product webpage