antibody

Anti-GPX4 Antibody [JU11-31]

ET1706-45TR

20 uL

99 USD

monoclonal

domestic rabbit

nonconjugated

JU11-31

human, mouse, rat, zebrafish

western blot, immunohistochemistry - paraffin section

Anti-GPX4 Antibody [JU11-31]

Human,Mouse,Rat,Zebrafish

WB,IHC-P,IF-Cell

Non-conjugated

Synthetic peptide within Human GPX4 aa 23-72 / 197.

SwissProt: P36969 Human;SwissProt: O70325 Mouse;SwissProt: P36970 Rat

Rabbit

JU11-31

IgG

20 uL

99 USD

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Liquid

Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.

Protein A affinity purified.

Recombinant Rabbit monoclonal Antibody

HeLa cell lysate, A549 cell lysate, HepG2 cell lysate, U-937 cell lysate, HEK-293 cell lysate, mouse testis tissue lysate, rat testis tissue lysate, mouse kidney tissue lysate, rat kidney tissue lysate, mouse brain tissue lysate, rat brain tissue lysate, zebrafish tissue lysates, HEK-293, rat epididymis tissue, human kidney tissue, mouse testis tissue, MCF-7, PC-3M, LOVO.

Predicted band size: 22 kDa

Mitochondrion, Cytoplasm.

1 mg/mL.

WB: 1:10,000 ;IHC-P: 1:200-1:1,000 ;IF-Cell: 1:50-1:200

ET1706-45_2.jpg

Western blot analysis of GPX4 on different lysates with Rabbit anti-GPX4 antibody (ET1706-45) at 1/10,000 dilution and competitor's antibody at 1/10,000 dilution. Lane 1: HeLa cell lysate (20 µg/Lane) Lane 2: A549 cell lysate (20 µg/Lane) Lane 3: HepG2 cell lysate (20 µg/Lane) Lane 4: U-937 cell lysate (20 µg/Lane) Lane 5: HEK-293 cell lysate (20 µg/Lane) Lane 6: Mouse testis tissue lysate (20 µg/Lane) Lane 7: Rat testis tissue lysate (20 µg/Lane) Lane 8: Mouse kidney tissue lysate (20 µg/Lane) Lane 9: Rat kidney tissue lysate (20 µg/Lane) Lane 10: Mouse brain tissue lysate (20 µg/Lane) Lane 11: Rat brain tissue lysate (20 µg/Lane) Predicted band size: 22 kDa Observed band size: 20 kDa Exposure time: 2 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1706-45) at 1/10,000 dilution and competitor's antibody at 1/10,000 dilution were used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:50,000 dilution was used for 1 hour at room temperature.

ET1706-45_3.jpg

Western blot analysis of GPX4 on zebrafish tissue lysates with Rabbit anti-GPX4 antibody (ET1706-45) at 1/500 dilution. Lysates/proteins at 10 µg/Lane. Predicted band size: 22 kDa Observed band size: 17 kDa Exposure time: 2 minutes; 12% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1706-45) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.

ET1706-45_4.jpg

Western blot analysis of GPX4 on different lysates with Rabbit anti-GPX4 antibody (ET1706-45) at 1/500 dilution. Lane 1: Hela-si NT cell lysate Lane 2: Hela-si GPX4 cell lysate Lysates/proteins at 10 µg/Lane. Predicted band size: 22 kDa Observed band size: 20,22 kDa Exposure time: 1 minutes 40 seconds; 4-20% SDS-PAGE gel. ET1706-45 was shown to specifically react with GPX4 in Hela-si NT cells. Weakened band was observed when Hela-si GPX4 sample was tested. Hela-si NT and Hela-si GPX4 samples were subjected to SDS-PAGE. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM in TBST for 1 hour at room temperature. The primary antibody (ET1706-45, 1/500) and Loading control antibody (Rabbit anti-GAPDH , ET1601-4, 1/10,000) were used in 5% BSA at room temperature for 2 hours. Goat Anti-rabbit IgG-HRP Secondary Antibody (HA1001) at 1:300,000 dilution was used for 1 hour at room temperature.

ET1706-45_5.jpg

Immunohistochemical analysis of paraffin-embedded rat epididymis tissue with Rabbit anti-GPX4 antibody (ET1706-45) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1706-45) at 1/200 dilution for 0.5 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

ET1706-45_6.jpg

Immunohistochemical analysis of paraffin-embedded human kidney tissue with Rabbit anti-GPX4 antibody (ET1706-45) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1706-45) at 1/200 dilution for 0.5 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

ET1706-45_7.jpg

Immunohistochemical analysis of paraffin-embedded mouse testis tissue with Rabbit anti-GPX4 antibody (ET1706-45) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1706-45) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

ET1706-45_8.jpg

Immunocytochemistry analysis of MCF-7 cells labeling GPX4 with Rabbit anti-GPX4 antibody (ET1706-45) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 10 minutes at 37 ℃, permeabilized with 0.05% Triton X-100 in PBS for 20 minutes, and then blocked with 2% negative goat serum for 30 minutes at room temperature. Cells were then incubated with Rabbit anti-GPX4 antibody (ET1706-45) at 1/50 dilution in 2% negative goat serum overnight at 4 ℃. Alexa Fluor®488 conjugate-Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.

ET1706-45_9.jpg

Immunocytochemistry analysis of PC-3M cells labeling GPX4 with Rabbit anti-GPX4 antibody (ET1706-45) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 10 minutes at 37 ℃, permeabilized with 0.05% Triton X-100 in PBS for 20 minutes, and then blocked with 2% negative goat serum for 30 minutes at room temperature. Cells were then incubated with Rabbit anti-GPX4 antibody (ET1706-45) at 1/50 dilution in 2% negative goat serum overnight at 4 ℃. Alexa Fluor®488 conjugate-Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.

ET1706-45_10.jpg

Immunocytochemistry analysis of LOVO cells labeling GPX4 with Rabbit anti-GPX4 antibody (ET1706-45) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 10 minutes at 37 ℃, permeabilized with 0.05% Triton X-100 in PBS for 20 minutes, and then blocked with 2% negative goat serum for 30 minutes at room temperature. Cells were then incubated with Rabbit anti-GPX4 antibody (ET1706-45) at 1/50 dilution in 2% negative goat serum overnight at 4 ℃. Alexa Fluor®488 conjugate-Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.