product summary
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company name :
HUABIO
product type :
antibody
product name :
Citrate synthetase
catalog :
ET1706-40
quantity :
100μl
price :
385.00 USD
clonality :
monoclonal
host :
domestic rabbit
conjugate :
nonconjugated
clone name :
JU80-30
reactivity :
human, mouse, rat
application :
western blot, flow cytometry, immunohistochemistry - paraffin section
more info or order :
image
image 1 :
Western blot analysis of Citrate synthetase on different lysates with Rabbit anti-Citrate synthetase antibody (ET1706-40) at 1/2,000 dilution.
Lane 1: HAP1-parental cell lysate
Lane 2: HAP1-Citrate synthetase KD cell lysate
Lysates/proteins at 10 µg/Lane.
Predicted band size: 51 kDa
Observed band size: 45 kDa
Exposure time: 14 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1706-40) at 1/2,000 dilution was used in K1803 at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
image 2 :
Western blot analysis of Citrate synthetase on different lysates with Rabbit anti-Citrate synthetase antibody (ET1706-40) at 1/1,000 dilution.
Lane 1: Rat kidney tissue lysates (20 µg/Lane)
Lane 2: Hela cell lysates
Lane 3: Mouse brain tissue lysates (20 µg/Lane)
Lysates/proteins at 10 µg/Lane.
Predicted band size: 52 kDa
Observed band size: 50 kDa
Exposure time: 2 minutes;
10% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1706-40) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 200,000 dilution was used for 1 hour at room temperature.
image 3 :
Immunohistochemical analysis of paraffin-embedded rat epididymis tissue with Rabbit anti-Citrate synthetase antibody (ET1706-40) at 1/50 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1706-40) at 1/50 dilution for 0.5 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
product information
SKU :
ET1706-40
Target name :
Citrate synthetase
Species reactivity :
Human,Mouse,Rat
Applications :
WB,IHC-P,FC
Conjugate :
Non-conjugated
Immunogen :
Synthetic peptide within Human Citrate synthetase aa 417-466 / 466.
Uniprot id :
O75390>SwissProt: O75390 Human;SwissProt: Q9CZU6 Mouse;SwissProt: Q8VHF5 Rat
Host :
Rabbit
Clone number :
JU80-30
Isotype :
IgG
Size :
100μl
List Price :
385.00 USD
Storage Buffer :
1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Form :
Liquid
Storage Instruction :
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.
Purity :
Protein A affinity purified.
Product type :
Recombinant Rabbit monoclonal Antibody
Positive control :
Rat kidney tissue lysate, Hela cell lysate, mouse brain tissue lysate, rat epididymis tissue, human tonsil tissue, human kidney tissue, 293T.
Molecular wt :
52 kDa
Subcellular location :
Mitochondrion matrix.
Concentration :
1 mg/mL.
Recommended dilutions :
WB: 1:1,000-1:2,000
;IHC-P: 1:50-1:100
;FC: 1:50-1:200
Advanced Validation :
Knockdown (KD)
Pic img4 :
https://storage.huabio.cn/huabio/productImg/ET1706-40_4.jpg
Pic legend4 :
Immunohistochemical analysis of paraffin-embedded human tonsil tissue with Rabbit anti-Citrate synthetase antibody (ET1706-40) at 1/50 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1706-40) at 1/50 dilution for 0.5 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Pic img5 :
https://storage.huabio.cn/huabio/productImg/ET1706-40_5.jpg
Pic legend5 :
Immunohistochemical analysis of paraffin-embedded human kidney tissue with Rabbit anti-Citrate synthetase antibody (ET1706-40) at 1/50 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1706-40) at 1/50 dilution for 0.5 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Pic img6 :
https://storage.huabio.cn/huabio/productImg/ET1706-40_6.jpg
Pic legend6 :
Flow cytometric analysis of 293T cells with Citrate synthetase antibody at 1/50 dilution (blue) compared with an unlabelled control (cells without incubation with primary antibody; red). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.
more info or order :
company information
HUABIO
Founded in 2007, HUABIO is dedicated to developing high-quality antibodies that advance innovation. We are passionate about the accuracy, efficiency, and consistency of our products. That is why we have invested in new production platforms, like recombinant rabbit monoclonals, alpaca nanobodies, and adopted aggressive QA standards to deliver cutting-edge antibodies with uncompromised quality.
We hope to see you at your next discovery!
We hope to see you at your next discovery!
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