USP14 | HUABIO ET1706-37 product information

product summary

company name :

HUABIO

product type :

antibody

product name :

USP14

catalog :

ET1706-37

quantity :

100μl

price :

385.00 USD

clonality :

monoclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

JU30-49

reactivity :

human, zebrafish

application :

western blot, flow cytometry, immunohistochemistry - paraffin section

more info or order :

HUABIO product webpage

image

image 1 :

Western blot analysis of USP14 on different lysates with Rabbit anti-USP14 antibody (ET1706-37) at 1/1,000 dilution. Lane 1: A549-si NT cell lysate Lane 2: A549-si USP14 cell lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 56 kDa Observed band size: 60 kDa Exposure time: 12 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1706-37) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

image 2 :

Western blot analysis of USP14 on different lysates with Rabbit anti-USP14 antibody (ET1706-37) at 1/500 dilution. Lane 1: K562 cell lysate Lane 2: Hela cell lysate Lysates/proteins at 10 µg/Lane. Predicted band size: 56 kDa Observed band size: 60 kDa Exposure time: 2 minutes; 10% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1706-37) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 200,000 dilution was used for 1 hour at room temperature.

image 3 :

Western blot analysis of USP14 on zebrafish lysates with Rabbit anti-USP14 antibody (ET1706-37) at 1/500 dilution. Lysates/proteins at 20 µg/Lane. Predicted band size: 56 kDa Observed band size: 60 kDa Exposure time: 2 minutes; 10% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1706-37) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:200,000 dilution was used for 1 hour at room temperature.

product information

SKU :

ET1706-37

Target name :

USP14

Species reactivity :

Human,Zebrafish

Applications :

WB,IF-Cell,IF-Tissue,IHC-P,FC

Conjugate :

Non-conjugated

Immunogen :

Recombinant protein within Human USP14 aa 351-494 / 494.

Uniprot id :

P54578>SwissProt: P54578 Human

Host :

Rabbit

Clone number :

JU30-49

Isotype :

IgG

Size :

100μl

List Price :

385.00 USD

Storage Buffer :

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Form :

Liquid

Storage Instruction :

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.

Purity :

Protein A affinity purified.

Product type :

Recombinant Rabbit monoclonal Antibody

Positive control :

K562 cell lysate, Hela cell lysate, zebrafish lysates, A431, HUVEC, LOVO, human colon carcinoma tissue, human kidney tissue, mouse prostate tissue, rat epididymis tissue, Jurkat.

Molecular wt :

Predicted band size: 56 kDa

Subcellular location :

Cytoplasm. Cell membrane.

Concentration :

1 mg/mL.

Recommended dilutions :

WB: 1:500-1:2,000 ;IF-Cell: 1:50-1:200 ;IHC-P: 1:50-1:200 ;FC: 1:50-1:100

Advanced Validation :

Knockdown (KD)

Pic img4 :

https://storage.huabio.cn/huabio/productImg/ET1706-37_4.jpg

Pic legend4 :

Immunocytochemistry analysis of A431 cells labeling USP14 with Rabbit anti-USP14 antibody (ET1706-37) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 10 minutes at 37 ℃, permeabilized with 0.05% Triton X-100 in PBS for 20 minutes, and then blocked with 2% negative goat serum for 30 minutes at room temperature. Cells were then incubated with Rabbit anti-USP14 antibody (ET1706-37) at 1/50 dilution in 2% negative goat serum overnight at 4 ℃. Alexa Fluor®488 conjugate-Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. Nuclear DNA was labelled in blue with DAPI.

Pic img5 :

https://storage.huabio.cn/huabio/productImg/ET1706-37_5.jpg

Pic legend5 :

Immunocytochemistry analysis of HUVEC cells labeling USP14 with Rabbit anti-USP14 antibody (ET1706-37) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 10 minutes at 37 ℃, permeabilized with 0.05% Triton X-100 in PBS for 20 minutes, and then blocked with 2% negative goat serum for 30 minutes at room temperature. Cells were then incubated with Rabbit anti-USP14 antibody (ET1706-37) at 1/50 dilution in 2% negative goat serum overnight at 4 ℃. Alexa Fluor®488 conjugate-Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. Nuclear DNA was labelled in blue with DAPI.

Pic img6 :

https://storage.huabio.cn/huabio/productImg/ET1706-37_6.jpg

Pic legend6 :

Immunocytochemistry analysis of LOVO cells labeling USP14 with Rabbit anti-USP14 antibody (ET1706-37) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 10 minutes at 37 ℃, permeabilized with 0.05% Triton X-100 in PBS for 20 minutes, and then blocked with 2% negative goat serum for 30 minutes at room temperature. Cells were then incubated with Rabbit anti-USP14 antibody (ET1706-37) at 1/50 dilution in 2% negative goat serum overnight at 4 ℃. Alexa Fluor®488 conjugate-Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. Nuclear DNA was labelled in blue with DAPI.

Pic img7 :

https://storage.huabio.cn/huabio/productImg/ET1706-37_7.jpg

Pic legend7 :

Immunohistochemical analysis of paraffin-embedded human colon carcinoma tissue with Rabbit anti-USP14 antibody (ET1706-37) at 1/50 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1706-37) at 1/50 dilution for 0.5 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Pic img8 :

https://storage.huabio.cn/huabio/productImg/ET1706-37_8.jpg

Pic legend8 :

Immunohistochemical analysis of paraffin-embedded human kidney tissue with Rabbit anti-USP14 antibody (ET1706-37) at 1/50 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1706-37) at 1/50 dilution for 0.5 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Pic img9 :

https://storage.huabio.cn/huabio/productImg/ET1706-37_9.jpg

Pic legend9 :

Immunohistochemical analysis of paraffin-embedded mouse prostate tissue with Rabbit anti-USP14 antibody (ET1706-37) at 1/50 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1706-37) at 1/50 dilution for 0.5 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Pic img10 :

https://storage.huabio.cn/huabio/productImg/ET1706-37_10.jpg

Pic legend10 :

Immunohistochemical analysis of paraffin-embedded rat epididymis tissue with Rabbit anti-USP14 antibody (ET1706-37) at 1/50 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1706-37) at 1/50 dilution for 0.5 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Pic img11 :

https://storage.huabio.cn/huabio/productImg/ET1706-37_11.jpg

Pic legend11 :

Flow cytometric analysis of USP14 was done on Jurkat cells. The cells were fixed, permeabilized and stained with the primary antibody (ET1706-37, 1/50) (red). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor®488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1,000 dilution for 30 minutes.Unlabelled sample was used as a control (cells without incubation with primary antibody; black).

more info or order :

HUABIO product webpage