product summary
Loading...
company name :
HUABIO
product type :
antibody
product name :
Involucrin
catalog :
ET1706-32
quantity :
100μl
price :
385.00 USD
clonality :
monoclonal
host :
domestic rabbit
conjugate :
nonconjugated
clone name :
JU30-27
reactivity :
human, rat
application :
western blot, flow cytometry, immunohistochemistry - paraffin section
more info or order :
image
image 1 :
Western blot analysis of Involucrin on human skin tissue lysates with Rabbit anti-Involucrin antibody (ET1706-32) at 1/500 dilution.
Lysates/proteins at 20 µg/Lane.
Predicted band size: 120 kDa
Observed band size: 120 kDa
Exposure time: 2 minutes;
8% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1706-32) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:200,000 dilution was used for 1 hour at room temperature.
image 2 :
Immunohistochemical analysis of paraffin-embedded human lung carcinoma tissue with Rabbit anti-Involucrin antibody (ET1706-32) at 1/50 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1706-32) at 1/50 dilution for 0.5 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
image 3 :
Flow cytometric analysis of A431 cells with Involucrin antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody.
product information
SKU :
ET1706-32
Target name :
Involucrin
Species reactivity :
Human,Rat
Applications :
WB,IHC-P,FC
Conjugate :
Non-conjugated
Immunogen :
Synthetic peptide within Human Involucrin aa 131-180 / 585.
Uniprot id :
P07476>SwissProt: P07476 Human;SwissProt: P48998 Rat
Host :
Rabbit
Clone number :
JU30-27
Isotype :
IgG
Size :
100μl
List Price :
385.00 USD
Storage Buffer :
1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Form :
Liquid
Storage Instruction :
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.
Purity :
Protein A affinity purified.
Product type :
Recombinant Rabbit monoclonal Antibody
Positive control :
Human skin tissue lysates, human lung carcinoma tissue, A431.
Molecular wt :
120 kDa
Subcellular location :
Cytoplasm
Concentration :
1 mg/mL.
Recommended dilutions :
WB: 1:500-1:2,000
;IHC-P: 1:50-1:200
;FC: 1:50-1:200
more info or order :
company information
HUABIO
Founded in 2007, HUABIO is dedicated to developing high-quality antibodies that advance innovation. We are passionate about the accuracy, efficiency, and consistency of our products. That is why we have invested in new production platforms, like recombinant rabbit monoclonals, alpaca nanobodies, and adopted aggressive QA standards to deliver cutting-edge antibodies with uncompromised quality.
We hope to see you at your next discovery!
We hope to see you at your next discovery!
browse more products
questions and comments