DDB1 | HUABIO ET1706-22 product information

product summary

company name :

HUABIO

product type :

antibody

product name :

DDB1

catalog :

ET1706-22

quantity :

100μl

price :

385.00 USD

clonality :

monoclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

JU32-35

reactivity :

human, mouse, rat

application :

western blot, immunoprecipitation, flow cytometry, immunohistochemistry - paraffin section

more info or order :

HUABIO product webpage

image

image 1 :

Western blot analysis of DDB1 on different lysates with Rabbit anti-DDB1 antibody (ET1706-22) at 1/2,000 dilution. Lane 1: MCF7 cell lysate Lane 2: HeLa cell lysate Lane 3: NIH/3T3 cell lysate Lane 4: Neuro-2a cell lysate Lane 5: PC-12 cell lysate Lane 6: C6 cell lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 127 kDa Observed band size: 127 kDa Exposure time: 2 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1706-22) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

image 2 :

Immunocytochemistry analysis of PC-12 cells labeling DDB1 with Rabbit anti-DDB1 antibody (ET1706-22) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-DDB1 antibody (ET1706-22) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (HA601187, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution.

image 3 :

Immunohistochemical analysis of paraffin-embedded human kidney tissue with Rabbit anti-DDB1 antibody (ET1706-22) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1706-22) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

product information

SKU :

ET1706-22

Target name :

DDB1

Species reactivity :

Human,Mouse,Rat

Applications :

WB,IF-Cell,IF-Tissue,IHC-P,IP,FC

Conjugate :

Non-conjugated

Immunogen :

Recombinant protein within Human DDB1 aa 1040-1140 / 1,140.

Uniprot id :

Q16531>SwissProt: Q16531 Human;SwissProt: Q3U1J4 Mouse;SwissProt: Q9ESW0 Rat

Host :

Rabbit

Clone number :

JU32-35

Isotype :

IgG

Size :

100μl

List Price :

385.00 USD

Storage Buffer :

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Form :

Liquid

Storage Instruction :

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.

Purity :

Protein A affinity purified.

Product type :

Recombinant Rabbit monoclonal Antibody

Positive control :

MCF7 cell lysate, HeLa cell lysate, NIH/3T3 cell lysate, Neuro-2a cell lysate, PC-12 cell lysate, C6 cell lysate, PC-12, human kidney tissue, mouse brain tissue, rat esophagus tissue.

Molecular wt :

Predicted band size: 127 kDa

Subcellular location :

Cytoplasm, Nucleus.

Concentration :

1 mg/mL.

Recommended dilutions :

WB: 1:2,000 ;IF-Cell: 1:50-1:200 ;IF-Tissue: 1:50-1:200 ;IHC-P: 1:1,000 ;IP: 1:10-1:50 ;FC: 1:1,000

Pic img4 :

https://storage.huabio.cn/huabio/productImg/ET1706-22_4.jpg

Pic legend4 :

Immunohistochemical analysis of paraffin-embedded mouse brain tissue with Rabbit anti-DDB1 antibody (ET1706-22) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1706-22) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Pic img5 :

https://storage.huabio.cn/huabio/productImg/ET1706-22_5.jpg

Pic legend5 :

Immunohistochemical analysis of paraffin-embedded rat esophagus tissue with Rabbit anti-DDB1 antibody (ET1706-22) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1706-22) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

more info or order :

HUABIO product webpage