CD11b | HUABIO ET1706-04-50UL product information

product summary

company name :

HUABIO

product type :

antibody

product name :

CD11b

catalog :

ET1706-04-50UL

quantity :

50μl

price :

205.00 USD

clonality :

monoclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

JU93-81

reactivity :

human

application :

western blot, immunoprecipitation, immunohistochemistry - paraffin section

more info or order :

HUABIO product webpage

image

image 1 :

Western blot analysis of CD11b on different lysates with Rabbit anti-CD11b antibody (ET1706-04) at 1/1,000 dilution. Lane 1: TF-1 cell lysate (10 µg/Lane) Lane 2: THP-1 cell lysate (15 µg/Lane) Lane 3: U-937 cell lysate (30 µg/Lane) Lane 4: Jurkat cell lysate (negative) (10 µg/Lane) Predicted band size: 127 kDa Observed band size: 170 kDa Exposure time: 1 minute 50 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1706-04) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

image 2 :

Fluorescence multiplex immunohistochemical analysis of the human gastric cancer (Formalin/PFA-fixed paraffin-embedded sections). Panel A: the merged image of anti-CD31 (M1511-8, red), anti-αSMA (ET1607-53, gray), anti-CD11b (ET1706-04, cyan), anti-panCK (HA601138, magenta) and anti-CD3 (HA720082, yellow) on human gastric cancer. Panel B: anti- CD31 stained on the endothelial cells. Panel C: anti-αSMA stained on cancer-associated fibroblasts and smooth muscle cells. Panel D: anti-CD11b stained on myeloid cells. Panel E: anti-panCK stained on cancer cells. Panel F: anti-CD3 stained on T cells. HRP Conjugated UltraPolymer Goat Polyclonal Antibody HA1119/HA1120 was used as a secondary antibody. The immunostaining was performed with the Sequential Immuno-staining Kit (IRISKit™MH010101, www.luminiris.cn). The section was incubated in five rounds of staining: in the order of M1511-8 (1/1,000 dilution), ET1607-53 (1/2,000 dilution), ET1706-04 (1/1,000 dilution), HA601138 (1/3,000 dilution), and HA720082 (1/500 dilution) for 20 mins at room temperature. Each round was followed by a separate fluorescent tyramide signal amplification system. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 30 mins at 95℃. DAPI (blue) was used as a nuclear counter stain. Image acquisition was performed with Olympus VS200 Slide Scanner.

image 3 :

Fluorescence multiplex immunohistochemical analysis of human gastric cancer (Formalin/PFA-fixed paraffin-embedded sections). Panel A: the merged image of anti-CD11b (ET1706-04, Red), anti-CD3 (HA720082, Green) and anti-CD31 (M1511-8, Yellow) on human gastric cancer. HRP Conjugated UltraPolymer Goat Polyclonal Antibody HA1119/HA1120 was used as a secondary antibody. The immunostaining was performed with the Sequential Immuno-staining Kit (IRISKit™MH010101, www.luminiris.cn). The section was incubated in three rounds of staining: in the order of ET1706-04 (1/1,000 dilution), HA720082 (1/500 dilution) and M1511-8 (1/1,000 dilution) for 20 mins at room temperature. Each round was followed by a separate fluorescent tyramide signal amplification system. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 30 mins at 95℃. DAPI (blue) was used as a nuclear counter stain. Image acquisition was performed with Zeiss Observer 7 Inverted Fluorescence Microscope.

product information

SKU :

ET1706-04-50UL

Target name :

CD11b

Species reactivity :

Human

Applications :

WB,IP,IHC-P,IF-Cell,IF-Tissue,mIHC

Conjugate :

Non-conjugated

Immunogen :

Synthetic peptide within Human CD11b 1103-1152 / 1152.

Uniprot id :

P11215>SwissProt: P11215 Human

Host :

Rabbit

Clone number :

JU93-81

Isotype :

IgG

Size :

50μl

List Price :

205.00 USD

Storage Buffer :

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Form :

Liquid

Storage Instruction :

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.

Purity :

Protein A affinity purified.

Product type :

Recombinant Rabbit monoclonal Antibody

Positive control :

TF-1 cell lysate, THP-1 cell lysate, U-937 cell lysate, THP-1, U-937, TF-1, human tonsil tissue, human lymph nodes tissue, human spleen tissue, human gastric cancer.

Molecular wt :

Predicted band size: 127 kDa

Subcellular location :

Cell membrane, Membrane raft.

Concentration :

1 mg/mL.

Recommended dilutions :

WB: 1:1,000 ;IF-Cell: 1:50-1:200 ;IF-Tissue: 1:50-1:200 ;IHC-P: 1:200-1:1,000 ;IP: Use at an assay dependent concentration. ;mIHC: 1:1,000

Advanced Validation :

Relative expression (RE)

Pic img4 :

https://storage.huabio.cn/huabio/productImg/ET1706-04_4.jpg

Pic legend4 :

Fluorescence multiplex immunohistochemical analysis of human cervical carcinoma (Formalin/PFA-fixed paraffin-embedded sections). Panel A: the merged image of anti-CD66b (HA500100, Green), anti-CD11b (ET1706-04, Red) and anti-CD68 (EM1901-95, Yellow) on human cervical carcinoma. HRP Conjugated UltraPolymer Goat Polyclonal Antibody HA1119/HA1120 was used as a secondary antibody. The immunostaining was performed with the Sequential Immuno-staining Kit (IRISKit™MH010101, www.luminiris.cn). The section was incubated in three rounds of staining: in the order of HA500100 (1/1,000 dilution), ET1706-04 (1/1,000 dilution) and EM1901-95 (1/3,000 dilution) for 20 mins at room temperature. Each round was followed by a separate fluorescent tyramide signal amplification system. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 30 mins at 95℃. DAPI (blue) was used as a nuclear counter stain. Image acquisition was performed with Zeiss Observer 7 Inverted Fluorescence Microscope.

Pic img5 :

https://storage.huabio.cn/huabio/productImg/ET1706-04_5.jpg

Pic legend5 :

Immunofluorescence analysis of paraffin-embedded human lymph nodes tissue labelling CD11 b (ET1706-04). The human lymph node section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes, blocked in 10% goat serum, and then incubated with ET1706-04 at 1/50 dilution , followed by iFluorTM 488 Conjugated Goat anti-rabbit IgG at 1:1000 dilution. Nuclear was stained with Hoechst 33258 at 1/5,000 dilution. Confocal images shows specific membrane staining of CD11b in human lymph node.

Pic img6 :

https://storage.huabio.cn/huabio/productImg/ET1706-04_6.jpg

Pic legend6 :

Immunofluorescence analysis of paraffin-embedded human spleen tissue labelling CD11 b (ET1706-04). The human spleen section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes, blocked in 10% goat serum, and then incubated with ET1706-04 at 1/50 dilution , followed by iFluorTM 488 Conjugated Goat anti-rabbit IgG at 1:1000 dilution. Nuclear was stained with Hoechst 33258 at 1/5,000 dilution. Confocal images shows specific membrane staining of CD11b in human spleen.

Pic img7 :

https://storage.huabio.cn/huabio/productImg/ET1706-04_7.jpg

Pic legend7 :

Immunocytochemistry analysis of THP-1 (positive) and Jurkat (negative) labeling CD11b with Rabbit anti-CD11b antibody (ET1706-04) at 1/100 dilution. Cells were fixed in 100% precooled methanol for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-CD11b antibody (ET1706-04) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (M1305-2, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution.

Pic img8 :

https://storage.huabio.cn/huabio/productImg/ET1706-04_8.jpg

Pic legend8 :

Immunocytochemistry analysis of U-937 cells labeling CD11b with Rabbit anti-CD11b antibody (ET1706-04) at 1/50 dilution. Cells were fixed in 100% methanol for 10 minutes, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes, and then blocked with 2% normal goat serum for 1 hour at 37℃. Cells were then incubated with Rabbit anti-CD11b antibody (ET1706-04) at 1/50 dilution in 2% negative goat serum overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (M1305-2, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) were used as the secondary antibody at 1/1,000 dilution.

Pic img9 :

https://storage.huabio.cn/huabio/productImg/ET1706-04_9.jpg

Pic legend9 :

Immunocytochemistry analysis of TF-1 cells labeling CD11b with Rabbit anti-CD11b antibody (ET1706-04) at 1/50 dilution. Cells were fixed in 100% methanol for 10 minutes, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes, and then blocked with 2% normal goat serum for 1 hour at 37℃. Cells were then incubated with Rabbit anti-CD11b antibody (ET1706-04) at 1/50 dilution in 2% negative goat serum overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (M1305-2, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) were used as the secondary antibody at 1/1,000 dilution.

Pic img10 :

https://storage.huabio.cn/huabio/productImg/ET1706-04_10.jpg

Pic legend10 :

Immunohistochemical analysis of paraffin-embedded human tonsil tissue with Rabbit anti-CD11b antibody (ET1706-04) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1706-04) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Pic img11 :

https://storage.huabio.cn/huabio/productImg/ET1706-04_11.jpg

Pic legend11 :

Immunohistochemical analysis of paraffin-embedded human lymph nodes tissue with Rabbit anti-CD11b antibody (ET1706-04) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1706-04) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Pic img12 :

https://storage.huabio.cn/huabio/productImg/ET1706-04_12.jpg

Pic legend12 :

Immunohistochemical analysis of paraffin-embedded human spleen tissue with Rabbit anti-CD11b antibody (ET1706-04) at 1/800 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1706-04) at 1/800 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

more info or order :

HUABIO product webpage