Eg5 | HUABIO ET1706-03 product information

product summary

company name :

HUABIO

product type :

antibody

product name :

Eg5

catalog :

ET1706-03

quantity :

100μl

price :

385.00 USD

clonality :

monoclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

JU83-16

reactivity :

human

application :

western blot, immunoprecipitation

more info or order :

HUABIO product webpage

image

image 1 :

Western blot analysis of Eg5 on different lysates with Rabbit anti-Eg5 antibody (ET1706-03) at 1/2,000 dilution. Lane 1: A431 cell lysate Lane 2: HEK-293 cell lysate Lysates/proteins at 10 µg/Lane. Predicted band size: 119 kDa Observed band size: 119 kDa Exposure time: Lane 1: 3 minutes; Lane 2: 59 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1706-03) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

image 2 :

Eg5 was immunoprecipitated from 0.2 mg HEK-293 cell lysate with ET1706-03 at 2 µg/10 µl beads. Western blot was performed from the immunoprecipitate using ET1706-03 at 1/2,000 dilution. Mouse Anti-Rabbit IgG kappa light chain secondary antibody (M1208-2) at 1/5,000 dilution was used for 1 hour at room temperature. Lane 1: HEK-293 cell lysate (input) Lane 2: ET1706-03 IP in HEK-293 cell lysate Lane 3: Rabbit IgG instead of ET1706-03 in HEK-293 cell lysate Blocking/Dilution buffer: 5% NFDM/TBST Exposure time: 3 minutes; ECL: K1801

product information

SKU :

ET1706-03

Target name :

Eg5

Species reactivity :

Human

Applications :

WB,IP

Conjugate :

Non-conjugated

Immunogen :

Recombinant protein within Human Eg5 aa 860-1,056 / 1,056.

Uniprot id :

P52732>SwissProt: P52732 Human

Host :

Rabbit

Clone number :

JU83-16

Isotype :

IgG

Size :

100μl

List Price :

385.00 USD

Storage Buffer :

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Form :

Liquid

Storage Instruction :

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.

Purity :

Protein A affinity purified.

Product type :

Recombinant Rabbit monoclonal Antibody

Positive control :

A431 cell lysate, HEK-293 cell lysate.

Molecular wt :

Predicted band size: 119 kDa

Subcellular location :

Cytoplasm, Cytoskeleton, Microtubule.

Concentration :

1 mg/mL.

Recommended dilutions :

WB: 1:2,000 ;IP: 1-2μg/sample

more info or order :

HUABIO product webpage