Histone H2A.X | HUABIO ET1705-97 product information

product summary

company name :

HUABIO

product type :

antibody

product name :

Histone H2A.X

catalog :

ET1705-97

quantity :

100μl

price :

385.00 USD

clonality :

monoclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

JM06-42

reactivity :

human, mouse, rat

application :

western blot, immunoprecipitation, immunohistochemistry - paraffin section

more info or order :

HUABIO product webpage

image

image 1 :

Western blot analysis of Histone H2A.X on different lysates with Rabbit anti-Histone H2A.X antibody (ET1705-97) at 1/1,000 dilution. Lane 1: Raji cell lysate Lane 2: NIH/3T3 cell lysate Lane 3: C6 cell lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 15 kDa Observed band size: 15 kDa Exposure time: 3 minutes; 15% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1705-97) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

image 2 :

Immunofluorescence staining of paraffin- embedded human pancreas tissue using anti-Histone H2A.X antibody.The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS, and then probed with ET1705-97 at 1/50 dilution for 10 hours at 4℃ and detected using Alexa Fluor® 488 conjugate-Goat anti-Rabbit IgG (H+L) Secondary Antibody at a dilution of 1:500 for 1 hour at room temperature.

image 3 :

Immunofluorescence staining of paraffin- embedded mouse testis tissue using anti-Histone H2A.X antibody.The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS, and then probed with ET1705-97 at 1/50 dilution for 10 hours at 4℃ and detected using Alexa Fluor® 488 conjugate-Goat anti-Rabbit IgG (H+L) Secondary Antibody at a dilution of 1:500 for 1 hour at room temperature.

product information

SKU :

ET1705-97

Target name :

Histone H2A.X

Species reactivity :

Human,Mouse,Rat

Applications :

WB,IF-Tissue,IHC-P,IP

Conjugate :

Non-conjugated

Immunogen :

Synthetic peptide within N-terminal human Histone H2A.X.

Uniprot id :

P16104>SwissProt: P16104 Human;SwissProt: P27661 Mouse;SwissProt: D3ZXP3 Rat

Host :

Rabbit

Clone number :

JM06-42

Isotype :

IgG

Size :

100μl

List Price :

385.00 USD

Storage Buffer :

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Form :

Liquid

Storage Instruction :

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.

Purity :

Protein A affinity purified.

Product type :

Recombinant Rabbit monoclonal Antibody

Positive control :

Raji cell lysate, MCF-7 cell lysate, human pancreas tissue, mouse testis tissue, rat brain tissue, human stomach carcinoma tissue, mouse pancreas tissue, rat pancreas tissue.

Molecular wt :

Predicted band size: 15 kDa

Subcellular location :

Nucleus. Chromosome.

Concentration :

1 mg/mL.

Recommended dilutions :

WB: 1:500-1:2000 ;IF-Tissue: 1:50-1:200 ;IHC-P: 1:1,000-1:4,000 ;IP: Use at an assay dependent concentration.

Pic img4 :

https://storage.huabio.cn/huabio/productImg/ET1705-97_4.jpg

Pic legend4 :

Immunofluorescence staining of paraffin- embedded rat brain tissue using anti-Histone H2A.X antibody.The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS, and then probed with ET1705-97 at 1/50 dilution for 10 hours at 4℃ and detected using Alexa Fluor® 488 conjugate-Goat anti-Rabbit IgG (H+L) Secondary Antibody at a dilution of 1:500 for 1 hour at room temperature.

Pic img5 :

https://storage.huabio.cn/huabio/productImg/ET1705-97_5.jpg

Pic legend5 :

Immunohistochemical analysis of paraffin-embedded human pancreas tissue with Rabbit anti-Histone H2A.X antibody (ET1705-97) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1705-97) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Pic img6 :

https://storage.huabio.cn/huabio/productImg/ET1705-97_6.jpg

Pic legend6 :

Immunohistochemical analysis of paraffin-embedded mouse testis tissue with Rabbit anti-Histone H2A.X antibody (ET1705-97) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1705-97) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Pic img7 :

https://storage.huabio.cn/huabio/productImg/ET1705-97_7.jpg

Pic legend7 :

Immunohistochemical analysis of paraffin-embedded rat brain tissue with Rabbit anti-Histone H2A.X antibody (ET1705-97) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1705-97) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Pic img8 :

https://storage.huabio.cn/huabio/productImg/ET1705-97_8.jpg

Pic legend8 :

Immunohistochemical analysis of paraffin-embedded human stomach carcinoma tissue with Rabbit anti-Histone H2A.X antibody (ET1705-97) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1705-97) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Pic img9 :

https://storage.huabio.cn/huabio/productImg/ET1705-97_9.jpg

Pic legend9 :

Immunohistochemical analysis of paraffin-embedded mouse pancreas tissue with Rabbit anti-Histone H2A.X antibody (ET1705-97) at 1/4,000 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1705-97) at 1/4,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Pic img10 :

https://storage.huabio.cn/huabio/productImg/ET1705-97_10.jpg

Pic legend10 :

Immunohistochemical analysis of paraffin-embedded rat pancreas tissue with Rabbit anti-Histone H2A.X antibody (ET1705-97) at 1/4,000 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1705-97) at 1/4,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

more info or order :

HUABIO product webpage