GCLM | HUABIO ET1705-87 product information

product summary

company name :

HUABIO

product type :

antibody

product name :

GCLM

catalog :

ET1705-87

quantity :

100μl

price :

385.00 USD

clonality :

monoclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

JM39-61

reactivity :

human, mouse, rat

application :

western blot, immunoprecipitation, immunohistochemistry - paraffin section, immunohistochemistry - frozen section

more info or order :

HUABIO product webpage

image

image 1 :

Western blot analysis of GCLM on different lysates with Rabbit anti-GCLM antibody (ET1705-87) at 1/1,000 dilution. Lane 1: A431 cell lysate Lane 2: NIH/3T3 cell lysate Lane 3: PC-12 cell lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 31 kDa Observed band size: 31 kDa Exposure time: 59 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1705-87) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

image 2 :

Western blot analysis of GCLM on different lysates with Rabbit anti-GCLM antibody (ET1705-87) at 1/2,000 dilution. Lane 1: HeLa cell lysate (15 µg/Lane) Lane 2: Mouse liver tissue lysate (20 µg/Lane) Lane 3: Rat liver tissue lysate (20 µg/Lane) Predicted band size: 31 kDa Observed band size: 31 kDa Exposure time: 3 minutes; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1705-87) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

image 3 :

Immunofluorescence analysis of frozen mouse hippocampus tissue labeling GCLM with Rabbit anti-GCLM antibody (ET1705-87). The tissues were blocked in 3% BSA for 30 minutes at room temperature, washed with PBS, and then probed with the primary antibody (ET1705-87, green) at 1/50 dilution overnight at 4℃, washed with PBS. Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) was used as the secondary antibody at 1/200 dilution. Nuclei were counterstained with DAPI (blue). Image acquisition was performed with KFBIO KF-FL-400 Scanner.

product information

SKU :

ET1705-87

Target name :

GCLM

Species reactivity :

Human,Mouse,Rat

Applications :

WB,IF-Tissue,IHC-P,IP,IHC-Fr

Conjugate :

Non-conjugated

Immunogen :

Recombinant protein within Human GCLM aa 225-274 / 274.

Uniprot id :

P48507>SwissProt: P48507 Human;SwissProt: O09172 Mouse;SwissProt: P48508 Rat

Host :

Rabbit

Clone number :

JM39-61

Isotype :

IgG

Size :

100μl

List Price :

385.00 USD

Storage Buffer :

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Form :

Liquid

Storage Instruction :

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.

Purity :

Protein A affinity purified.

Product type :

Recombinant Rabbit monoclonal Antibody

Positive control :

A431 cell lysate, NIH/3T3 cell lysate, PC-12 cell lysate, HeLa cell lysate, Mouse liver tissue lysate, Rat liver tissue lysate, human liver tissue, human lung tissue, mouse liver tissue, rat liver tissue.

Molecular wt :

Predicted band size: 31 kDa

Subcellular location :

Cytosol

Concentration :

1 mg/mL.

Recommended dilutions :

WB: 1:1,000-1:2,000 ;IF-Tissue: 1:50-1:200 ;IHC-P: 1:200-1:1,000 ;IP: Use at an assay dependent concentration. ;IHC-Fr: 1:50

Pic img4 :

https://storage.huabio.cn/huabio/productImg/ET1705-87_4.jpg

Pic legend4 :

Immunofluorescence analysis of frozen mouse cerebral cortex tissue labeling GCLM with Rabbit anti-GCLM antibody (ET1705-87). The tissues were blocked in 3% BSA for 30 minutes at room temperature, washed with PBS, and then probed with the primary antibody (ET1705-87, green) at 1/100 dilution overnight at 4℃, washed with PBS. Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) was used as the secondary antibody at 1/200 dilution. Nuclei were counterstained with DAPI (blue). Image acquisition was performed with KFBIO KF-FL-400 Scanner.

Pic img5 :

https://storage.huabio.cn/huabio/productImg/ET1705-87_5.jpg

Pic legend5 :

Immunohistochemical analysis of paraffin-embedded human liver tissue with Rabbit anti-GCLM antibody (ET1705-87) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1705-87) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Pic img6 :

https://storage.huabio.cn/huabio/productImg/ET1705-87_6.jpg

Pic legend6 :

Immunohistochemical analysis of paraffin-embedded human lung tissue with Rabbit anti-GCLM antibody (ET1705-87) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1705-87) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Pic img7 :

https://storage.huabio.cn/huabio/productImg/ET1705-87_7.jpg

Pic legend7 :

Immunohistochemical analysis of paraffin-embedded mouse liver tissue with Rabbit anti-GCLM antibody (ET1705-87) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1705-87) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Pic img8 :

https://storage.huabio.cn/huabio/productImg/ET1705-87_8.jpg

Pic legend8 :

Immunohistochemical analysis of paraffin-embedded rat liver tissue with Rabbit anti-GCLM antibody (ET1705-87) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1705-87) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

more info or order :

HUABIO product webpage