PFKFB3 | HUABIO ET1705-66 product information

product summary

company name :

HUABIO

product type :

antibody

product name :

PFKFB3

catalog :

ET1705-66

quantity :

100μl

price :

385.00 USD

clonality :

monoclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

JM43-43

reactivity :

human, mouse, rat

application :

western blot, immunoprecipitation, flow cytometry, immunohistochemistry - paraffin section

more info or order :

HUABIO product webpage

image

image 1 :

All lanes: Western blot analysis of PFKFB3 with anti-PFKFB3 antibody [JM43-43] (ET1705-66) at 1/500 dilution. Lane 1: Wild-type Hela whole cell lysate. Lane 2: PFKFB3 knockout Hela whole cell lysate. ET1705-66 was shown to specifically react with PFKFB3 in wild-type Hela cells. No band was observed when PFKFB3 knockout sample was tested. Wild-type and PFKFB3 knockout samples were subjected to SDS-PAGE. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM in TBST for 1 hour at room temperature. The primary Anti-PFKFB3 antibody (ET1705-66, 1/500) and Anti-HSP90 antibody (ET1605-56, 1/10,000) were used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG H&L (HRP) Secondary Antibody (HA1001) at 1:200,000 dilution was used for 1 hour at room temperature. Cell lysate was provided by Ubigene Biosciences (Ubigene Biosciences Co., Ltd., Guangzhou, China).

image 2 :

Western blot analysis of PFKFB3 on different lysates with Rabbit anti-PFKFB3 antibody (ET1705-66) at 1/1,000 dilution. Lane 1: HeLa cell lysate Lane 2: PC-12 cell lysate Lane 3: A431 cell lysate Lane 4: A549 cell lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 60 kDa Observed band size: 60 kDa Exposure time: 2 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1705-66) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:100,000 dilution was used for 1 hour at room temperature.

image 3 :

Immunohistochemical analysis of paraffin-embedded human kidney tissue with Rabbit anti-PFKFB3 antibody (ET1705-66) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1705-66) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

product information

SKU :

ET1705-66

Target name :

PFKFB3

Species reactivity :

Human,Mouse,Rat

Applications :

WB,IF-Cell,IHC-P,FC,IP,IF-Tissue

Conjugate :

Non-conjugated

Immunogen :

Recombinant protein within Human PFKFB3 aa 260-520 / 520.

Uniprot id :

Q16875>SwissProt: Q16875 Human;SwissProt: O35552 Rat;SwissProt: Q3U3S6 Mouse

Host :

Rabbit

Clone number :

JM43-43

Isotype :

IgG

Size :

100μl

List Price :

385.00 USD

Storage Buffer :

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Form :

Liquid

Storage Instruction :

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.

Purity :

Protein A affinity purified.

Product type :

Recombinant Rabbit monoclonal Antibody

Positive control :

HeLa cell lysate, PC-12 cell lysate, A431 cell lysate, A549 cell lysate, human kidney tissue, A549, Hela, rat kidney tissue, mouse colon tissue, A431.

Molecular wt :

Predicted band size: 60 kDa

Subcellular location :

Cytosol. Nucleoplasm.

Concentration :

1 mg/mL.

Recommended dilutions :

WB: 1:500-1:1,000 ;IF-Cell: 1:50-1:200 ;IHC-P: 1:1,000 ;FC: 1:50-1:100 ;IP: Use at an assay dependent concentration. ;IF-Tissue: 1:200

Advanced Validation :

Knockout (KO)

Pic img4 :

https://storage.huabio.cn/huabio/productImg/ET1705-66_4.jpg

Pic legend4 :

PFKFB3 was immunoprecipitated from 0.2 mg HeLa cell lysate with ET1705-66 at 2 µg/10 µl beads. Western blot was performed from the immunoprecipitate using ET1705-66 at 1/1,000 dilution. Anti-Rabbit IgG for IP Nano-secondary antibody (NBI01H) at 1/5,000 dilution was used for 1 hour at room temperature. Lane 1: HeLa cell lysate (input) Lane 2: ET1705-66 IP in HeLa cell lysate Lane 3: Rabbit IgG instead of ET1705-66 in HeLa cell lysate Blocking/Dilution buffer: 5% NFDM/TBST Exposure time: 2 minutes; ECL: K1802

Pic img5 :

https://storage.huabio.cn/huabio/productImg/ET1705-66_5.jpg

Pic legend5 :

ICC staining of PFKFB3 in A549 cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (ET1705-66, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue).

Pic img6 :

https://storage.huabio.cn/huabio/productImg/ET1705-66_6.jpg

Pic legend6 :

ICC staining of PFKFB3 in Hela cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (ET1705-66, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue).

Pic img7 :

https://storage.huabio.cn/huabio/productImg/ET1705-66_7.jpg

Pic legend7 :

Immunohistochemical analysis of paraffin-embedded rat kidney tissue using anti-PFKFB3 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1705-66, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Pic img8 :

https://storage.huabio.cn/huabio/productImg/ET1705-66_8.jpg

Pic legend8 :

Immunohistochemical analysis of paraffin-embedded mouse colon tissue using anti-PFKFB3 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1705-66, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Pic img9 :

https://storage.huabio.cn/huabio/productImg/ET1705-66_9.jpg

Pic legend9 :

Flow cytometric analysis of PFKFB3 was done on A431 cells. The cells were fixed, permeabilized and stained with the primary antibody (ET1705-66, 1/50) (red). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated Goat anti-Rabbit IgG Secondary antibody at 1/1000 dilution for 30 minutes.Unlabelled sample was used as a control (cells without incubation with primary antibody; black).

more info or order :

HUABIO product webpage