PGC1 beta | HUABIO ET1705-65 product information

product summary

company name :

HUABIO

product type :

antibody

product name :

PGC1 beta

catalog :

ET1705-65

quantity :

100μl

price :

385.00 USD

clonality :

monoclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

JM33-24

reactivity :

human, rat

application :

western blot, immunoprecipitation, flow cytometry

more info or order :

HUABIO product webpage

image

image 1 :

Western blot analysis of PGC1 beta on different lysates with Rabbit anti-PGC1 beta antibody (ET1705-65) at 1/1,000 dilution. Lane 1: SK-Br-3 cell lysate Lane 2: HeLa cell lysate Lane 3: HepG2 cell lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 113 kDa Observed band size: 113 kDa Exposure time: 24 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1705-65) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

image 2 :

Western blot analysis of PGC1 beta on rat spleen tissue lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ET1705-65, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:200,000 dilution was used for 1 hour at room temperature.

image 3 :

Flow cytometric analysis of PGC1 beta was done on HL-60 cells. The cells were fixed, permeabilized and stained with the primary antibody (ET1705-65, 1/50) (red). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated Goat anti-Rabbit IgG Secondary antibody at 1/1000 dilution for 30 minutes.Unlabelled sample was used as a control (cells without incubation with primary antibody; black).

product information

SKU :

ET1705-65

Target name :

PGC1 beta

Species reactivity :

Human,Rat

Applications :

WB,IP,FC

Conjugate :

Non-conjugated

Immunogen :

Synthetic peptide within Human PGC1 beta aa 974-1023 / 1023.

Uniprot id :

Q86YN6>SwissProt: Q86YN6 Human;SwissProt: Q811R2 Rat

Host :

Rabbit

Clone number :

JM33-24

Isotype :

IgG

Size :

100μl

List Price :

385.00 USD

Storage Buffer :

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Form :

Liquid

Storage Instruction :

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.

Purity :

Protein A affinity purified.

Product type :

Recombinant Rabbit monoclonal Antibody

Positive control :

SK-Br-3 cell lysate, HeLa cell lysate, HepG2 cell lysate, rat spleen tissue lysates, HL-60.

Molecular wt :

Predicted band size: 113 kDa

Subcellular location :

Nucleus.

Concentration :

1 mg/mL.

Recommended dilutions :

WB: 1:500-1:2,000 ;FC: 1:50-1:200 ;IP: 1:10-1:50

more info or order :

HUABIO product webpage