product summary
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company name :
HUABIO
product type :
antibody
product name :
PDHB
catalog :
ET1705-57
quantity :
100μl
price :
385.00 USD
clonality :
monoclonal
host :
domestic rabbit
conjugate :
nonconjugated
clone name :
JM66-33
reactivity :
human, mouse, rat
application :
western blot, immunoprecipitation, flow cytometry, immunohistochemistry - paraffin section
more info or order :
image
image 1 :
Western blot analysis of PDHB on different lysates with Rabbit anti-PDHB antibody (ET1705-57) at 1/2,000 dilution.
Lane 1: HeLa cell lysate (20 µg/Lane)
Lane 2: HepG2 cell lysate (20 µg/Lane)
Lane 3: C2C12 cell lysate (20 µg/Lane)
Lane 4: L6 cell lysate (20 µg/Lane)
Lane 5: Mouse heart tissue lysate (40 µg/Lane)
Lane 6: Rat heart tissue lysate (40 µg/Lane)
Predicted band size: 39 kDa
Observed band size: 34 kDa
Exposure time: 8 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1705-57) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
image 2 :
Western blot analysis of PDHB on different lysates with Rabbit anti-PDHB antibody (ET1705-57) at 1/2,000 dilution.
Lane 1: HAP1-parental cell lysate
Lane 2: HAP1-PDHB KD cell lysate
Lysates/proteins at 10 µg/Lane.
Predicted band size: 39 kDa
Observed band size: 34 kDa
Exposure time: 6 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1705-57) at 1/2,000 dilution was used in K1803 at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
image 3 :
Immunohistochemical analysis of paraffin-embedded human brain tissue with Rabbit anti-PDHB antibody (ET1705-57) at 1/200 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1705-57) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
product information
SKU :
ET1705-57
Target name :
PDHB
Species reactivity :
Human,Mouse,Rat
Applications :
WB,IHC-P,FC,IP
Conjugate :
Non-conjugated
Immunogen :
Synthetic peptide within Human PDHB aa 310-359 / 359.
Uniprot id :
P11177>SwissProt: P11177 Human;SwissProt: Q9D051 Mouse;SwissProt: P49432 Rat
Host :
Rabbit
Clone number :
JM66-33
Isotype :
IgG
Size :
100μl
List Price :
385.00 USD
Storage Buffer :
1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Form :
Liquid
Storage Instruction :
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.
Purity :
Protein A affinity purified.
Product type :
Recombinant Rabbit monoclonal Antibody
Positive control :
HeLa cell lysate, HepG2 cell lysate, C2C12 cell lysate, L6 cell lysate, Mouse heart tissue lysate, Rat heart tissue lysate, human brain tissue, human heart tissue, mouse brain tissue, mouse heart tissue, rat brain tissue, rat heart tissue, Hela.
Molecular wt :
Predicted band size: 39 kDa
Subcellular location :
Mitochondrion matrix.
Concentration :
1 mg/mL.
Recommended dilutions :
WB: 1:2,000
;IHC-P: 1:200-1:1,000
;FC: 1:50-1:100
;IP: Use at an assay dependent concentration.
Advanced Validation :
Knockdown (KD)
Pic img4 :
https://storage.huabio.cn/huabio/productImg/ET1705-57_4.jpg
Pic legend4 :
Immunohistochemical analysis of paraffin-embedded human heart tissue with Rabbit anti-PDHB antibody (ET1705-57) at 1/1,000 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1705-57) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Pic img5 :
https://storage.huabio.cn/huabio/productImg/ET1705-57_5.jpg
Pic legend5 :
Immunohistochemical analysis of paraffin-embedded mouse brain tissue with Rabbit anti-PDHB antibody (ET1705-57) at 1/200 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1705-57) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Pic img6 :
https://storage.huabio.cn/huabio/productImg/ET1705-57_6.jpg
Pic legend6 :
Immunohistochemical analysis of paraffin-embedded mouse heart tissue with Rabbit anti-PDHB antibody (ET1705-57) at 1/200 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1705-57) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Pic img7 :
https://storage.huabio.cn/huabio/productImg/ET1705-57_7.jpg
Pic legend7 :
Immunohistochemical analysis of paraffin-embedded rat brain tissue with Rabbit anti-PDHB antibody (ET1705-57) at 1/200 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1705-57) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Pic img8 :
https://storage.huabio.cn/huabio/productImg/ET1705-57_8.jpg
Pic legend8 :
Immunohistochemical analysis of paraffin-embedded rat heart tissue with Rabbit anti-PDHB antibody (ET1705-57) at 1/200 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1705-57) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Pic img9 :
https://storage.huabio.cn/huabio/productImg/ET1705-57_9.jpg
Pic legend9 :
Flow cytometric analysis of Hela cells with PDHB antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black).
more info or order :
company information
HUABIO
Founded in 2007, HUABIO is dedicated to developing high-quality antibodies that advance innovation. We are passionate about the accuracy, efficiency, and consistency of our products. That is why we have invested in new production platforms, like recombinant rabbit monoclonals, alpaca nanobodies, and adopted aggressive QA standards to deliver cutting-edge antibodies with uncompromised quality.
We hope to see you at your next discovery!
We hope to see you at your next discovery!
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