product summary
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company name :
HUABIO
product type :
antibody
product name :
Anti-Phospho-MLKL (S345) Antibody [JM92-37]
catalog :
ET1705-51TR
quantity :
20 uL
price :
99 USD
clonality :
monoclonal
host :
domestic rabbit
conjugate :
nonconjugated
clone name :
JM92-37
reactivity :
mouse
application :
western blot, immunohistochemistry - paraffin section
more info or order :
product information
SKU :
ET1705-51TR
Target name :
Anti-Phospho-MLKL (S345) Antibody [JM92-37]
Species reactivity :
Mouse
Applications :
WB,IHC-P
Conjugate :
Non-conjugated
Immunogen :
Synthetic phospho-peptide corresponding to residues surrounding Ser345 of mouse MLKL aa 314-363 / 472.
Uniprot id :
SwissProt: Q9D2Y4 Mouse
Host :
Rabbit
Clone number :
JM92-37
Isotype :
IgG
Size :
20 uL
List Price :
99 USD
Storage Buffer :
1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Form :
Liquid
Storage Instruction :
Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.
Purity :
Protein A affinity purified.
Product type :
Recombinant Rabbit monoclonal Antibody
Positive control :
L929 treated with 20μM Z-VAD for 3.5 hours then added 100nM SM-164 and 20ng/ml TNF-α for 3 hours cell lysate, mouse spleen tissue, mouse lung tissue, mouse liver tissue, mouse colon tissue.
Molecular wt :
Predicted band size: 54 kDa
Subcellular location :
Cell membrane, Cytoplasm, Nucleus.
Concentration :
1 mg/mL.
Recommended dilutions :
WB:1:1,000
;IHC-P: 1:50-1:100
Pic img4 :
ET1705-51_2.jpg
Pic img5 :
Immunohistochemical analysis of paraffin-embedded mouse spleen tissue using anti-Phospho-MLKL (S345) antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1705-51, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Pic legend5 :
ET1705-51_3.jpg
Pic legend6 :
Immunohistochemical analysis of paraffin-embedded mouse lung tissue using anti-Phospho-MLKL (S345) antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1705-51, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Pic img7 :
ET1705-51_4.jpg
Pic img8 :
Immunohistochemical analysis of paraffin-embedded mouse liver tissue using anti-Phospho-MLKL (S345) antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1705-51, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Pic legend8 :
ET1705-51_5.jpg
Pic legend9 :
Immunohistochemical analysis of paraffin-embedded mouse colon tissue using anti-Phospho-MLKL (S345) antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1705-51, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
more info or order :
company information
HUABIO
Founded in 2007, HUABIO is dedicated to developing high-quality antibodies that advance innovation. We are passionate about the accuracy, efficiency, and consistency of our products. That is why we have invested in new production platforms, like recombinant rabbit monoclonals, alpaca nanobodies, and adopted aggressive QA standards to deliver cutting-edge antibodies with uncompromised quality.
We hope to see you at your next discovery!
We hope to see you at your next discovery!
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